Evaluation of nitric oxide scavenging activity of certain spices in vitro:: A preliminary study
Evaluation of nitric oxide scavenging activity of certain spices in vitro:: A preliminary study
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DOI：
10.1002/food.200390061
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发表时间：
2003-08-01
期刊：
影响因子：
--
通讯作者：
Babu, K
中科院分区：
文献类型：
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作者：
Baliga, MS;Jagetia, GC;Babu, K
以硝普钠为NO供体，研究了几种常用香料的植物提取物对一氧化氮(NO)水平的可能调节作用。所测试的大部分提取物对NO有直接的清除作用，并表现出显著的清除活性，清除活性的顺序如下：小茴香(水)>；柑橘(水)>；九里香(种子，水)>；九里香(叶，水)>；姜黄(水)>；九里香(叶，二氯甲烷：甲醇)>；薄荷(水)>；薄荷(水)>；银杏>；九里香(二氯甲烷：甲醇)>；生姜(水)>；薄荷(水)>；姜黄(乙醇)>；九里香(种子，二氯甲烷：甲醇)。所有被评价的提取物都表现出剂量依赖的NO清除活性。小茴香水提物在浓度为62.5µg/m L时清除能力最强，为79.75%，而阳性对照银杏叶水提物的清除能力为36.22%。本研究结果提示，这些香料可能是清除NO和调节过量产生NO及其氧化产物过氧化亚硝酸根引起的病理状态的有效的新型治疗剂。
The plant extracts of some commonly used spices were examined for their possible regulatory effect on nitric oxide (NO) levels using sodium nitroprusside as a NO donor in vitro. Most of the extracts tested demonstrated direct scavenging of NO and exhibited significant activity and the potency of scavenging activity was in the following order: Foeniculum vulgare (aqueous) > Citrus limettiodes > Murraya koenigii (seed, aqueous) > Murraya koenigii (leaf, aqueous) > Curcuma aromatica (aqueous) > Murraya koenigii (leaf, dichloromethane:methanol) > Mentha arvensis (chloroform) > Mentha arvensis (aqueous) > Curcuma longa > Gingko biloba > Foeniculum vulgare (dichloromethane:methanol) > Zingiber officinale (aqueous) > Curcuma aromatica (ethanolic) > Murraya koenigii (seed, dichloromethane:methanol). All the evaluated extracts exhibited a dose-dependent NO scavenging activity. The aqueous extract of Foeniculum vulgare showed a greatest NO scavenging effect of 79.75% at 62.5 mug/mL as compared to the positive control, Gingko biloba where 36.22% scavenging was observed at similar concentration. The present results suggest that these spices might be potent and novel therapeutic agents for scavenging of NO and the regulation of pathological conditions caused by excessive generation of NO and its oxidation product, peroxynitrite.