Detection of covalent DNA-protein complexes: the adenovirus DNA-terminal protein complex and HeLa DNA-protein complexes.
Detection of covalent DNA-protein complexes: the adenovirus DNA-terminal protein complex and HeLa DNA-protein complexes.
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共价 DNA-蛋白质复合物的检测：腺病毒 DNA 末端蛋白质复合物和 HeLa DNA-蛋白质复合物。
DOI：
10.1101/sqb.1979.043.01.082
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
G. D. Pearson
中科院分区：
文献类型：
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作者：
D. Coombs;A. Robinson;J. W. Bodnar;C. J. Jones;G. D. Pearson
材料和方法细胞。 HeLa $3 细胞使用补充有 7% 胎牛血清 (FCS) 的 F-13 培养基 (GIBCO) 以 3 x 10 s 细胞/ml 的浓度悬浮培养。在含有 5 mM HEPES (pH 7.2) 和 2% FCS 的无磷酸盐 F-13 中，用浓度为 1 mCi/L 的 [32p] 正磷酸盐（新英格兰核；无载体）标记细胞 24 小时。
MATERIALS AND METHODSCells. HeLa $3 cells were grown in suspension culture at a concentration of 3 x 10 s cells/ml using Medium F-13 (GIBCO) supplemented with 7% fetal calf serum (FCS). Cells were labeled for 24 hours with [32p] orthophosphate (New England Nuclear; carrier-free) at a concentration of 1 mCi/liter in phosphate-free F-13 containing 5 mM HEPES (pH 7.2) and 2% FCS.