Histological Assessment of Tumor Tissue Samples via the Mirror Image Method
Histological Assessment of Tumor Tissue Samples via the Mirror Image Method
复制标题
通过镜像法对肿瘤组织样本进行组织学评估
DOI：
10.1089/bio.2014.0093
复制
发表时间：
2015-02-01
影响因子：
1.6
通讯作者：
Chen, Kexin
中科院分区：
文献类型：
--
作者：
Li, Haixin;Guo, Yan;Chen, Kexin
人体组织生物库已成为加速中国癌症基础和转化生物医学研究的关键平台。维持足够数量的肿瘤细胞对于各种癌症研究至关重要。确保冷冻储存组织标本的高质量是一项至关重要的要求。然而，不同的肿瘤位置和不同的肿瘤组织切除方法可能导致库存组织标本中肿瘤细胞的数量不同。因此，评估肿瘤细胞含量的有效方法对于生物库中的组织样本至关重要。在本研究中，镜像法被用来评估存储的肿瘤组织中的肿瘤细胞的数量的六种常见的癌症类型，包括实体和中空器官癌。所有组织均储存在天津医科大学肿瘤研究所和医院（TMUCIH）。组织学评估由病理学家进行，病理学家对冷冻储存的样品的镜像切片进行肿瘤百分比的形态学诊断，所述样品用苏木精和伊红（H&E）染色。结果表明，实体性脏器癌的肿瘤百分率高于空腔性脏器癌（χ 2 = 17.11，P
Human tissue biorepositories have become key platforms for the acceleration of basic and translational biomedical research on cancer in China. The maintenance of sufficient amounts of tumor cells is critical for a wide variety of cancer studies. Ensuring the high quality of frozen stored tissue specimens is a crucial requirement. However, different tumor locations and various methods of tumor tissue removal can lead to variable numbers of tumor cells from banked tissue specimens. Thus, an effective method to assess the tumor cell content is essential for tissue samples in biobanks. In the present study, the mirror image method was used to evaluate the amount of tumor cells in stored tumor tissues of six common cancer types, including solid and hollow organ cancers. All tissues were stored in the Tianjin Medical University Cancer Institute and Hospital (TMUCIH). Histological assessment was performed by pathologists who conducted morphological diagnoses of tumor percentage on mirror image sections of frozen stored samples that were stained with hematoxylin and eosin (H&E). Results showed that the tumor percentage of solid organ cancers was higher than that of hollow organ cancers (chi(2)=17.11, p