Characterization of Non-coding Regions in B Mating Loci of Agrocybe salicacola Groups: Target Sites for B Mating Type Identification
Characterization of Non-coding Regions in B Mating Loci of Agrocybe salicacola Groups: Target Sites for B Mating Type Identification
复制标题
杨树菇群体 B 交配位点非编码区的特征：B 交配类型识别的目标位点
DOI：
10.1007/s00284-017-1247-5
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发表时间：
2017-06-01
影响因子：
2.6
通讯作者：
Zhao, YongChang
中科院分区：
文献类型：
--
作者：
Chen, Weimin;Chai, Hongmei;Zhao, YongChang
柳生茶树菇是一种美味的可栽培蘑菇。因此，了解这一物种的内在特征，特别是阐明交配基因的组成和分离规律具有重要意义。本研究利用两个亲和的B交配位点，对A.通过序列分析和同源性分析，发现两个保守的信息素受体基因可能失去了交配活性，这是通过与其它蘑菇的比较而确定的。在交配位点的保守区，部分插入/缺失片段造成非编码区的多态性，根据可变区的扩增谱可以区分不同交配型的单核菌株，说明交配位点在A.柳珊瑚利用担子菌交配位点的非编码区片段建立B交配型分子标记尚属首次。
Agrocybe salicacola is a delicious and cultivable mushroom. It is important to understand this species' inherent characteristics, especially to elucidate the constitution and segregation of mating genes. In this study, two compatible B mating loci in strain YAASM0711 of A. salicacola were cloned from the monokaryons, and sequence and phylogeny analyses showed two conserved genes encoding pheromone receptors maybe lost mating activity, which determined by comparing with those of other mushrooms. In the conserved regions of mating loci, partial insertion/deletion fragments made non-coding regions posses polymorphisms, and monokaryotic strains of different mating types were distinguished from each other according to the amplification profile of variable regions, which suggested mating loci were integrally assigned to offspring strains during mitosis in A. salicacola. As our known, it is the first to develop molecular markers for B mating-type identification using variable non-coding fragments of mating loci in basidiomycetes.