The P0 gene of Sugarcane yellow leaf virus encodes an RNA silencing suppressor with unique activities
The P0 gene of Sugarcane yellow leaf virus encodes an RNA silencing suppressor with unique activities
复制标题
DOI：
10.1016/j.virol.2008.10.034
复制
发表时间：
2009-02-05
期刊：
影响因子：
3.7
通讯作者：
Albert, Henrik H.
中科院分区：
文献类型：
--
作者：
Mangwende, Tichaona;Wang, Ming-Li;Albert, Henrik H.
甘蔗黄叶病毒（Sugarcane yellow leaf virus，SCYLV）P0是Poleroviruses高度异源蛋白的一员，是转录后基因沉默（PTGS）的抑制因子，具有其他PO蛋白所没有的活性。PO蛋白在以前测试poleroviruses（甜菜西部黄化病毒和葫芦蚜传黄化病毒），抑制本地，但不是系统性的，PTGS诱导的正义GFP和反向重复GF使用其F盒样结构域介导Argonaute 1蛋白的不稳定。我们现在报道，SCYLV PO蛋白不仅抑制由正义GFP和反向重复GF诱导的本氏烟草中的局部PTGS，而且还在浸润的叶片中引发剂量依赖性细胞死亡表型并抑制系统正义GFP-PTGS。删除SCYLV PO的前15个N-末端氨基酸残基可消除对局部和全身PTGS的抑制以及对细胞死亡的诱导。相反，当15个C-末端氨基酸残基被删除时，只有系统性PTGS和细胞死亡丢失。我们的结论是，15个C-末端氨基酸残基区域的SCYLV PO是必要的抑制系统性PTGS和诱导细胞死亡，但不需要抑制局部PTGS。(C)2008年爱思唯尔公司All rights reserved.
The Sugarcane yellow leaf virus (SCYLV) P0, a member of the highly heterologous proteins of poleroviruses, is a suppressor of posttranscriptional gene silencing (PTGS) and has additional activities not seen in other PO proteins. The PO protein in previously tested poleroviruses (Beet western yellows virus and Cucurbit aphidborne yellows virus), suppresses local, but not systemic, PTGS induced by both sense GFP and inverted repeat GF using its F-box-like domain to mediate destabilization of the Argonaute1 protein. We now report that the SCYLV PO protein not only suppressed local PTGS induced by sense GFP and inverted repeat GF in Nicotiana benthamiana, but also triggered a dosage dependent cell death phenotype in infiltrated leaves and suppressed systemic sense GFP-PTGS. Deletion of the first 15 N-terminal amino acid residues of SCYLV PO abolished suppression of both local and systemic PTGS and the induction of cell death. In contrast, only systemic PTGS and cell death were lost when the 15 C-terminal amino acid residues were deleted. We conclude that the 15 C-terminal amino acid residue region of SCYLV PO is necessary for suppressing systemic PTGS and inducing cell death, but is not required for suppression of local PTGS. (C) 2008 Elsevier Inc. All rights reserved.