Genetic basis for nongroupable Neisseria meningitidis
Genetic basis for nongroupable Neisseria meningitidis
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DOI：
10.1086/374740
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发表时间：
2003-05-15
影响因子：
6.4
通讯作者：
Stephens, DS
中科院分区：
文献类型：
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作者：
Dolan-Livengood, JM;Miller, YK;Stephens, DS
不可分组的脑膜炎奈瑟菌可能占从人类携带者鼻咽部回收的脑膜炎球菌分离株的三分之一或更多。不可分组性的遗传基础是在一项基于人群的研究中获得的分离株中确定的，其中来自无症状携带者的 194 株脑膜炎球菌分离株中有 60 株（30.9%）不可分组。 42% 的不可分组分离株与血清群 Y ET-508/ST-23 克隆复合株相关，这是研究人群中最常见的可分组携带者分离株。不可分组的分离株均被 10% 正常人血清迅速杀死。详细研究了 6 种 ET-508/ST-23 复合菌株和 25 种其他遗传多样性不可分组脑膜炎球菌的荚膜基因座。未发现血清群 A 或新型荚膜生物合成基因。不可分组的分离株是基因血清群 Y、B 或 C 分离株，不表达荚膜，但与群体中发现的可分组分离株相关（I 类）；由于荚膜生物合成基因的插入元件相关缺失而导致荚膜缺陷（II 类）；或缺乏所有荚膜基因并形成与脑膜炎球菌疾病无关的独特遗传簇的分离株（III 类）。
Nongroupable Neisseria meningitidis may constitute one-third or more of meningococcal isolates recovered from the nasopharynx of human carriers. The genetic basis for nongroupability was determined in isolates obtained from a population-based study in which 60 (30.9%) of 194 meningococcal isolates from asymptomatic carriers were not groupable. Forty-two percent of nongroupable isolates were related to serogroup Y ET-508/ ST-23 clonal complex strains, the most common groupable carrier isolate from the study population. Nongroupable isolates were all rapidly killed by 10% normal human serum. The capsule loci of 6 of the ET-508/ ST-23 complex strains and of 25 other genetically diverse nongroupable meningococci were studied in detail. Serogroup A or novel capsule biosynthesis genes were not found. Nongroupable isolates were genetically serogroup Y, B, or C isolates that did not express capsule but were related to groupable isolates found in the population ( class I); capsule deficient because of insertion element-associated deletions of capsule biosynthesis genes (class II); or isolates that lacked all capsule genes and formed a distinct genetic cluster not associated with meningococcal disease (class III).