Transfer and stability of drug resistance plasmids inEscherichia coli K12
Transfer and stability of drug resistance plasmids inEscherichia coli K12
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耐药质粒在大肠杆菌K12中的转移和稳定性
DOI：
10.1007/bf02011590
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发表时间：
1984
影响因子：
3.6
通讯作者：
J. Howard Slater
中科院分区：
文献类型：
--
作者：
Peter C. Gowland;J. Howard Slater
在混合连续流培养物和悬浮于池水中的透析囊中进行了含有相容性质粒TP 120（Inc N）和R1（Inc FII）或不相容性质粒TP 125（Inc B）和TP 113（Inc B）的大肠杆菌菌株对之间的交配实验。在连续流培养和池塘水中，携带相容质粒TP 120和R1的菌株之间容易证明质粒转移。在携带质粒TP 125和TP 113的菌株的混合培养物中，仅在连续流动培养系统中观察到转移。菌株E.质粒TP 120和R1的共含聚集体显示在碳限制条件下以0.1小时-1的生长速率在选择用于维持两种质粒的药物存在下连续培养5个月是稳定的。在含有质粒聚集体的菌株中，观察到基因剂量效应与对药物的抗性水平有关，所述药物的抗性由两种质粒编码。恒化器实验表明，在E.大肠杆菌，最初含有质粒TP 120和质粒R1。
Mating experiments between pairs of strains ofEscherichia colicontaining either the compatible plasmids TP120 (Inc N) and R1 (Inc FII) or the incompatible plasmids TP125 (Inc B) and TP113 (Inc B) were undertaken in mixed continuous-flow cultures and in dialysis sacs suspended in pond water. Plasmid transfer was readily demonstrated between strains carrying compatible plasmids TP120 and R1 in both continuous-flow culture and pond water. In mixed cultures of strains carrying plasmids TP125 and TP113, transfer was only observed in continuous-flow culture systems. Strains ofE. colicontaining aggregates of plasmids TP120 and R1 were shown to be stable over 5 months continuous cultivation under carbon limited conditions at a growth rate of 0.1 hours−1in the presence of drugs which select for the maintenance of both plasmids. In the strains containing plasmid aggregates, a gene dosage effect was observed with respect to the levels of resistance to drugs whose resistance was encoded by both plasmids. Chemostat experiments showed that no cointegrate plasmids were found from the strains ofE. coliinitially containing both plasmid TP120 and plasmid R1.