Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments
Establishment of cell lines that exhibit pluripotency from miniature swine periodontal ligaments
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DOI：
10.1016/j.archoralbio.2007.04.009
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发表时间：
2007-10-01
影响因子：
3
通讯作者：
Tamura, Masato
中科院分区：
文献类型：
--
作者：
Ibi, Miho;Ishisaki, Akira;Tamura, Masato
目的：牙周膜（PDL）是由异质细胞组成的纤维结缔组织，其中包括PDL成纤维细胞。目前尚不清楚PDL成纤维细胞群中的细胞是否保留分化为其他细胞类型的潜能。设计图：在本研究中，克隆细胞系，来自克劳小型猪PDLs，通过基因转染人端粒酶逆转录酶，并进行了表征。结果如下：这些细胞系，表示为TesPDL 1 -4，具有PDL成纤维细胞，其显示成纤维细胞形态并表达前胶原α 1（I）、骨桥蛋白、骨膜蛋白和碱性磷酸酶mRNA。在特定的培养条件下，TesPDL 3细胞还具有表达CD 31、血管内皮钙粘蛋白、血管性血友病因子、骨钙素和形成细胞外矿化结节的能力。结论：我们的数据表明，TesPDL 3细胞具有在培养物中表达成纤维细胞、血管内皮细胞和成骨细胞的多种表型的独特性质。(C)2007爱思唯尔有限公司保留所有权利。
Objective: The periodontal ligament (PDL) is a fibrous connective tissue composed of heterogeneous cell types, including PDL fibroblasts. It is not clear whether cells within the PDL fibroblast population retain the potency to differentiate into other cell types. Design: In the present study, clonal cell lines, derived from Clawn miniature swine PDLs, were established by gene transfection for a human telomerase reverse transcriptase, and characterized. Results: These cell lines, denoted TesPDL1-4, had PDL fibroblasts that showed fibroblastic morphology and expressed procollagen alpha 1(I), osteopontin, periostin and alkaline phosphatase mRNA. Under the specific culture conditions, TesPDL3 cells also have the ability to express CD31, vascular endothelial cadherin, von Willebrand factor, osteocalcin, and to form extracellular mineralized nodules. Conclusions: Our data indicate that TesPDL3 cells have unique properties of expressing several phenotype of fibroblasts, vascular endothelial cells and osteoblasts in cultures. (C) 2007 Elsevier Ltd. All rights reserved.