THE YEAST SW14 PROTEIN CONTAINS A MOTIF PRESENT IN DEVELOPMENTAL REGULATORS AND IS PART OF A COMPLEX INVOLVED IN CELL-CYCLE-DEPENDENT TRANSCRIPTION
THE YEAST SW14 PROTEIN CONTAINS A MOTIF PRESENT IN DEVELOPMENTAL REGULATORS AND IS PART OF A COMPLEX INVOLVED IN CELL-CYCLE-DEPENDENT TRANSCRIPTION
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DOI：
10.1038/342830a0
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发表时间：
1989-12-14
期刊：
影响因子：
64.8
通讯作者：
HERSKOWITZ, I
中科院分区：
文献类型：
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作者：
ANDREWS, BJ;HERSKOWITZ, I
酿酒酵母hogene的转录，该基因编码一种位点特异性核酸内切酶，启动细胞类型转换（参见文献综述）。1,2)，被限制在Gl晚期细胞周期的短窗口（参考文献3,4）。ho上游区域的一个重复元件（细胞周期盒，CCB）和两个调节蛋白SWI4和SWI6是ho5的细胞周期依赖性表达所必需的。生化实验已经确定了一种因子，CCBF（细胞周期盒因子），它与CCB元素结合，可能在ho的细胞周期调节中起关键作用。swi4和swi6基因是形成CCBF-DNA复合体所必需的。在这里，我们报道了sw4基因的核苷酸序列，并表明它包含两个保守的sw6 - cdclo基序，在出芽酵母中观察到sw6 - cdclo基序，在萌发酵母中观察到sw6 - cdclo基序，在萌发酵母中观察到sw6 - cdclo基序，在发育过程中观察到sw6 - cdc10基因，在果蝇中观察到cdc10基因，在秀丽隐杆线虫中观察到cdc10基因-12和glp-1基因- 10 -12。我们通过在凝胶转移实验中使用SWI4蛋白的抗体证明该蛋白存在于CCBF-DNA复合物中。
TRANSCRIPTION of theHOgene ofSaccharomyces cerevisiae, which encodes a site-specific endonuclease that initiates cell-type switching (reviewed in refs. 1,2), is restricted to a short window of the cell cycle in late Gl (refs 3,4). A repeated element in the upstream region ofHO(the cell-cycle box, CCB) and two regulatory proteins, SWI4 and SWI6, are required for cell-cycle-dependent expression ofHO5,6. Biochemical experiments have identified a factor, CCBF (cell-cycle box factor), that binds to the CCB elements7and that presumably plays a key part in cell-cycle regulation ofHO. TheSWI4andSWI6genes are required for formation of the CCBF-DNA complex. Here we report the nucleotide sequence of theSWI4gene and show that it contains two copies of the conserved SWI6-cdclO motif observed inSWI6of budding yeast8, theSchizosaccharomyces pombe cdc10gene required for progression through G19, theDrosophila Notchgene, and in theCaenorhabditis elegans lin-12andglp-1genes10–12. We demonstrate by using antibodies to the SWI4 protein in gel-shift assays that the protein is present in the CCBF–DNA complex.