Reproducibility of prefrontal γ-aminobutyric acid measurements with J-edited spectroscopy
Reproducibility of prefrontal γ-aminobutyric acid measurements with J-edited spectroscopy
复制标题
DOI：
10.1002/nbm.1662
复制
发表时间：
2011-11-01
影响因子：
2.9
通讯作者：
Marenco, Stefano
中科院分区：
文献类型：
--
作者：
Geramita, Matthew;van der Veen, Jan Willem;Marenco, Stefano
γ-氨基丁酸 (GABA) 是人脑的主要抑制性神经递质，GABA 能功能障碍与多种神经精神疾病有关。最近的 MRS 技术已经可以量化体内 GABA 浓度，因此可以提供生物学相关信息。很少有报告正式描述这些技术的再现性，场强、采集和处理参数的差异可能会导致测量的 GABA 值存在较大差异。在这里，我们使用 J 编辑的单体素光谱方法测量 3 T 下前扣带皮层 (ACC) 和右额叶白质 (rFWM) 中的 GABA + 大分子 (GABA +)。我们测量了从 10 名健康志愿者获得的两次重复扫描的受试者内变异系数 (CVw) 和组内相关系数 内部开发的处理程序用于定量 GABA + 和其他主要代谢物。此外，通过将光谱体素分割为脑脊液、灰质和白质，并采用线性回归技术将代谢值外推到纯灰质和白质，我们确定了 ACC 和 rFWM 中灰质和白质之间的代谢差异。在 ACC 和 rFWM 中，GABAR/肌酸、GABA +/H2O、GABA +、肌酸、部分共编辑的谷氨酸 + 谷氨酰胺 (Glx)/肌酸、部分共编辑的 Glx 和 N-乙酰天冬氨酸 (NAA)/肌酸的 CVw 值均低于 12%。外推至纯灰质和纯白质后，所有代谢物的 CVw 值均低于 16%。我们发现灰质和白质之间的 GABA +/肌酸、GABA +、肌酸、部分共同编辑的 Glx 和 NAA/肌酸的代谢物比率分别为 0.88 +/- 0.21（标准差）、1.52 +/- 0.32、1.77 +/- 0.4、2.69 +/- 0.74 和 0.70 +/- 0.05。这项研究验证了一种可重复的脑代谢物定量方法，并提供了灰质/白质差异的信息，这对于解释临床人群的结果可能很重要。 John Wiley & Sons, Ltd 于 2011 年出版。
gamma-Aminobutyric acid (GABA) is the chief inhibitory neurotransmitter of the human brain, and GABA-ergic dysfunction has been implicated in a variety of neuropsychiatric disorders. Recent MRS techniques have allowed the quantification of GABA concentrations in vivo, and could therefore provide biologically relevant information. Few reports have formally characterized the reproducibility of these techniques, and differences in field strength, acquisition and processing parameters may result in large differences in measured GABA values. Here, we used a J-edited, single-voxel spectroscopy method of measurement of GABA + macromolecules (GABA +) in the anterior cingulate cortex (ACC) and right frontal white matter (rFWM) at 3 T. We measured the coefficient of variation within subjects (CVw) and intra-class correlation coefficients on two repeated scans obtained from 10 healthy volunteers with processing procedures developed in-house for the quantification of GABA + and other major metabolites. In addition, by segmenting the spectroscopic voxel into cerebrospinal fluid, gray matter and white matter, and employing a linear regression technique to extrapolate metabolite values to pure gray and white matter, we determined metabolite differences between gray and white matter in ACC and rFWM. CVw values for GABAR/creatine, GABA +/H2O, GABA +, creatine, partially co-edited glutamate + glutamine (Glx)/creatine, partially co-edited Glx and N-acetylaspartic acid (NAA)/creatine were all below 12% in both ACC and rFWM. After extrapolation to pure gray and pure white matter, CVw values for all metabolites were below 16%. We found metabolite ratios between gray and white matter for GABA +/creatine, GABA +, creatine, partially co-edited Glx and NAA/creatine to be 0.88 +/- 0.21 (standard deviation), 1.52 +/- 0.32, 1.77 +/- 0.4, 2.69 +/- 0.74 and 0.70 +/- 0.05, respectively. This study validates a reproducible method for the quantification of brain metabolites, and provides information on gray/white matter differences that may be important in the interpretation of results in clinical populations. Published in 2011 by John Wiley & Sons, Ltd.