Cumulibacter manganitolerans gen. nov., sp. nov., isolated from sludge of a manganese mine
Cumulibacter manganitolerans gen. nov., sp. nov., isolated from sludge of a manganese mine
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甘露糖积杆菌 (Cumulibacter manganitolerans)
DOI：
10.1099/ijsem.0.002004
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Gejiao Wang
中科院分区：
文献类型：
--
作者：
Jing Huang;Jiahong Li;Min Cao;Shuijiao Liao;Gejiao Wang
从某锰矿排污口的污泥中分离到一株革兰氏阳性放线菌，命名为2- 36 T。16 S rRNA基因系统发育分析表明，菌株2- 36 T为土皮菌科的一个单系，与玫瑰红菌KLBMP 1279 T（93.8%）、岩生芽生球菌BC 444 T（93.1%）和水管土皮菌BMG 801 T（92.8%）的亲缘关系最近。异C16：0、异C15：0、异C17：1！6c，iso-C14：0.和总和特征3（C16：1！6c和/或C16：1！7 c）为主要脂肪酸（> 5%），MK-9（H4）、MK-8（H4）和MK-9（H6）为醌。细胞壁含有作为诊断性二氨基酸的meso-二氨基庚二酸，DNA G+C含量为70.1mol%。极性脂质为二磷脂酰甘油、磷脂酰甲基乙醇胺、磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰肌醇甘露糖苷、一种未知糖脂、一种未知氨基磷脂和两种未知磷脂。与近缘菌株相比，菌株2- 36 T具有明显的系统发育特征，苯乙酸同化产物阳性，α-葡萄糖苷酶阴性，C17：1！6c和iso-C14：0为主要脂肪酸。根据多相分析，菌株2- 36 T代表土皮菌科中的一个新属的一个新种，其名称为耐锰累积杆菌.gen. nov.，sp. nov.的报道。耐锰累积杆菌的模式菌株为2- 36 T（=CCTCC AA 2016026 T = DSM. 103787 T）。
A Gram-stain-positive actinobacterium, designated as strain 2-36T, was isolated from sludge of a sewage outlet in a.manganese mine. Phylogenetic analysis of 16S rRNA genes assigned strain 2-36T in a single lineage in the family.Geodermatophilaceae and closely related to Modestobacter roseus KLBMP 1279T (93.8 % similarity), Blastococcus saxobsidens.BC444T (93.1 %) and Geodermatophilus aquaeductus BMG801T (92.8 %). It contained iso-C16 : 0, iso-C15 : 0, C17 : 1!6c, iso-C14 : 0.and summed feature 3 (C16 : 1!6c and/or C16 : 1!7c) as the major fatty acids (>5 %), with MK-9(H4), MK-8(H4) and MK-9(H6) as.the quinones. The cell wall contained meso-diaminopimelic acid as the diagnostic diamino acid and the DNA G+C content.was 70.1 mol%. The polar lipids were diphosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine,.phosphatidylinositol, phosphatidylinositol mannoside, an unknown glycolipid, an unknown aminophospholipid and two.unknown phospholipids. Compared to closely related strains, strain 2-36T showed distinguishing characteristics, such as.the distinct phylogenetic lineage, positive result for phenylacetic acid assimilation and negative result for a-glucosidase and.having C17 : 1!6c and iso-C14 : 0 as the major fatty acids. On the basis of the polyphasic analyses, strain 2-36T represents a.novel species of a new genus within the family Geodermatophilaceae, for which the name Cumulibacter manganitolerans.gen. nov., sp. nov. is proposed. The type strain of ’Cumulibacter manganitolerans’ is 2-36T (=CCTCC AA 2016026T=DSM.103787T).