Complete nucleotide sequences of Bacillus plasmids pUB110dB, pRBH1 and its copy mutants
Complete nucleotide sequences of Bacillus plasmids pUB110dB, pRBH1 and its copy mutants
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DOI：
10.1007/bf00330534
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
R. Muller;T. Ano;T. Imanaka;S. Aiba
中科院分区：
文献类型：
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作者：
R. Muller;T. Ano;T. Imanaka;S. Aiba
缺失质粒pRBH1 （1.5 MDa，卡那霉素耐药，Kmr）和pUB110dB （1.5 MDa, Kmr）分别从pTB913 （2.9 MDa, Kmr，分离自嗜热杆菌）和pUB110 （3.0 MDa, Kmr，分离自金黄色葡萄球菌）中获得。测定这些缺失质粒的核苷酸序列。pRBH1和pUB110dB的复制起始区分别含有63个碱基对的反向重复和一个大的开放阅读框，编码RepB蛋白（235个氨基酸残基）。除了倒置重复序列中心的一个碱基外，核苷酸序列彼此完全相同。从pRBH1中获得两个拷贝数突变质粒pRBHC3和pRBHC7。突变点位于RepB蛋白编码区的不同位置（pRBHC3为Gly - Asp， pRBHC7为Gly - Glu）。RepB蛋白参与了这些质粒的拷贝数控制。
The deletion plasmids, pRBH1 (1.5 MDa, kanamycin resistance, Kmr) and pUB110dB (1.5 MDa, Kmr), were obtained from pTB913 (2.9 MDa, Kmr, isolated from a thermophilic bacillus) and pUB110 (3.0 MDa, Kmr, fromStaphylococcus aureus), respectively. All the nucleotide sequences of these deletion plasmids were determined. Replication origin regions of pRBH1 and pUB110dB contained, respectively, 63 base-pair inverted repeat and a large open reading frame, encoding RepB protein (235 amino acid residues). The nucleotide sequences were identical to each other except for one base in the center of the inverted repeat. Two copy number mutant plasmids, pRBHC3 and pRBHC7, were obtained from pRBH1. The mutation points were located at different positions in the RepB protein coding region (Gly to Asp for pRBHC3 and Gly to Glu for pRBHC7). RepB protein was shown to be involved in the copy number control of these plasmids.