Association of enteric shedding of bovine torovirus (Breda virus) and other enteropathogens with diarrhea in veal calves
Association of enteric shedding of bovine torovirus (Breda virus) and other enteropathogens with diarrhea in veal calves
复制标题
DOI：
10.2460/ajvr.2003.64.485
复制
发表时间：
2003-04-01
影响因子：
1
通讯作者：
Saif, LJ
中科院分区：
文献类型：
--
作者：
Hoet, AE;Smiley, J;Saif, LJ
目的-确定小牛到达小牛场时以及小牛到达后的前 35 天内定期检测牛环病毒 (BoTV) 的患病率、粪便排泄模式以及与腹泻的关系。动物 - 62 头小牛。程序-通过使用 ELISA 和反向检测，对到达后第 0、4、14 和 35 天收集的粪便样本进行 BoTV 检测。 转录酶聚合酶链反应（RT-PCR）测定。使用血凝抑制测定法分析从第 0 天和第 35 天收集的血液中获得的配对血清样品中的 BoTV 抗体。还对粪便样本进行了其他肠道病原体筛查，包括轮状病毒、冠状病毒和隐孢子虫属。结果 - 通过 ELISA 和 RT-PCR 检测，在 62 头犊牛中的 15 头 (24%) 中检测到 BoTV 排泄物，第 4 天排泄量达到峰值。观察到 BoTV 排泄量与腹泻之间存在显着的独立关联。另外，小牛掉毛！ 2种肠道病原体比犊牛排泄物更容易出现腹泻：1种病原体。到达时血清阴性或 BoTV 抗体滴度较低（小于或等于 1:10 血凝抑制单位）的犊牛血清转化为 BoTV（滴度增加 > 4 倍）；这些犊牛比抵达时 BoTV 血清呈阳性的犊牛更容易传播病毒。结论和临床相关性 - BoTV 的传播与新生小牛抵达农场后第一周的腹泻密切相关。这些数据证明 BoTV 是新生小牛的重要病原体。
Objective-To determine the prevalence, fecal shedding pattern, and association of bovine torovirus (BoTV) with diarrhea in veal calves at time of arrival and periodically throughout the first 35 days after their arrival on a veal farm.Animals-62 veal calves.Procedure-Fecal samples collected on days 0, 4, 14, and 35 after arrival were tested for BoTV by use of ELISA and reverse transcriptase-polymerase chain reaction (RT-PCR) assay. Paired serum samples obtained from blood collected on days 0 and 35 were analyzed for BoTV antibodies with a hemagglutination inhibition assay. Fecal samples were also screened for other enteric pathogens, including rotavirus, coronavirus, and Cryptosporidium spp.Results-Fecal shedding of BoTV was detected in 15 of 62 (24%) calves by use of ELISA and RT-PCR assay, with peak shedding on day 4. A significant independent association between BoTV shedding and diarrhea was observed. In addition, calves shedding ! 2 enteric pathogens were more likely to have diarrhea than calves shedding : 1 pathogen. Calves that were seronegative or had low antibody titers against BoTV (less than or equal to 1:10 hemagglutination inhibition units) at arrival seroconverted to BoTV (> 4-fold increase in titer); these calves were more likely to shed virus than calves that were seropositive against BoTV at arrival.Conclusions and Clinical Relevance-Shedding of BoTV was strongly associated with diarrhea in neonatal veal calves during the first week after arrival at the farm. These data provide evidence that BoTV is an important pathogen of neonatal veal calves.