Colonization of neural allografts by host microglial cells: Relationship to graft neovascularization
Colonization of neural allografts by host microglial cells: Relationship to graft neovascularization
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DOI：
10.1016/s0963-6897(97)00030-4
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发表时间：
1997-05-01
影响因子：
3.3
通讯作者：
Streit, WJ
中科院分区：
文献类型：
--
作者：
Pennell, NA;Streit, WJ
为了阐明小胶质细胞在神经同种异体移植物中的功能作用，我们将完整的和去除小胶质细胞和内皮细胞的E14神经细胞悬浮液移植到Sprague-Dawley大鼠的完整纹状体中。在长达30天的移植后时间后，使用Griffoilia simplicifolia B-4 isolectin（小胶质细胞和血管的标记物）对脑内同种异体移植物进行组织化学分析，我们的研究结果表明，完整和耗尽的悬浮移植物在新血管形成和小胶质细胞定植方面发展相同，在两种类型的移植物中，小胶质细胞在任何血管出现之前出现，移植物血管形成的主要阶段发生在移植后第7天和第10天之间，并且新血管形成在第21天完成，如通过定量图像分析所揭示的，小胶质细胞，移植后30天，移植物内的小胶质细胞已经呈现出静息的成年小胶质细胞的完全分枝的表型特征。在移植物发育和血管形成期间，小胶质细胞经常被观察到靠近向内生长的血管和血管芽，总之，我们的研究表明，移植物的小胶质细胞定植和移植物血管化的发生独立于供体来源的小胶质细胞和内皮细胞，并表明移植物内的绝大多数小胶质细胞和血管是宿主来源的，我们假设宿主小胶质细胞侵入同种异体移植物在促进移植物新生血管化中发挥积极作用。(C)1997年爱思唯尔科学公司
In order to illuminate functional roles of microglial cells within neural allografts, we have transplanted both whole and microglial and endothelial cell-depleted E14 neural cell suspensions into the intact striatum of Sprague-Dawley rats, Following posttransplantation times of up to 30 days, the intrastrial allografts were analyzed histochemically using the Griffoilia simplicifolia B-4 isolectin, a marker for both microglia and blood vessels, Our results indicate that both whole and depleted suspension grafts develop identically in terms of neovascularization and microglial colonization, In both types of transplants microglial cells appeared before any blood vessels were apparent, The main phase of graft vascularization occurred between days 7 and 10 posttransplantation and neovascularization was complete by day 21, as revealed by quantitative image analysis, Microglial cells, which were present as ameboid cells during early posttransplantation times, underwent continuing cell differentiation with time that paralleled graft vascular development, By 30 days posttransplantation microglia within the grafts had assumed the fully ramified phenotype characteristic of resting adult microglia, During graft development and vascularization, microglia were often seen in close proximity to ingrowing blood vessels and vascular sprouts, In conclusion, our study has shown that microglial colonization of grafts and graft vascularization occurs independent of donor-derived microglial and endothelial cells, and suggests that the great majority of microglia and vessels within the graft are host derived, We hypothesize that the host microglia invading the allografts play an active role in promoting graft neovascularization. (C) 1997 Elsevier Science Inc.