Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.
Sequence of U1 RNA from Drosophila melanogaster: implications for U1 secondary structure and possible involvement in splicing.
复制标题
果蝇 U1 RNA 序列：对 U1 二级结构的影响以及可能参与剪接。
作者：
Stephen M. Mount;J. Steitz
来自培养的果蝇细胞 (Kc) 的 U1 RNA 通过其被人类狼疮患者的抗 (U1)RNP 抗体识别为 RNP 的能力来鉴定。其序列主要是通过对 RNA 分子的直接分析推导出来的，然后通过对通过与果蝇 U1 RNA 杂交分离的基因组克隆进行 DNA 序列测定来确认。果蝇 U1 RNA 序列与人类 U1 RNA 的一致性为 72%。核苷酸 3-11 与 hnRNA 中供体 (5') 剪接点的整个共有序列以及受体 (3') 共有序列的一部分互补，是完全保守的。然而，假定仅与受体连接相互作用的核苷酸 14-21 有所不同。果蝇 U1 序列与脊椎动物 U1 序列的比较允许特定的二级结构模型优于其他模型。这些结果与 U1 snRNP 参与剪接的假设一致，但建议对模型进行具体修改，详细说明剪接反应期间 U1 RNA 和 hnRNA 之间的分子相互作用。
U1 RNA from cultured Drosophila melanogaster cells (Kc) was identified by its ability to be recognized, as an RNP, by anti-(U1)RNP antibodies from human lupus patients. Its sequence was deduced largely from direct analysis of the RNA molecule and then confirmed by DNA sequence determinations on a genomic clone isolated by hybridization to Drosophila U1 RNA. The Drosophila U1 RNA sequence exhibits 72% agreement with human U1 RNA. Nucleotides 3-11, which are complementary to the entire consensus sequence for donor (5') splice junctions in hnRNA, and to part of the acceptor (3') consensus, are exactly conserved. However, nucleotides 14-21, postulated to interact only with acceptor junctions, differ. Comparison of the Drosophila U1 sequence with vertebrate U1 sequences allows a particular secondary structure model to be preferred over others. These results are consistent with the hypothesis that U1 snRNPs are involved in splicing, but suggest specific modifications of the model detailing molecular interactions between U1 RNA and hnRNA during the splicing reaction.