The effect of the hydrophilic/hydrophobic ratio of polymeric micelles on their endocytosis pathways into cells.
The effect of the hydrophilic/hydrophobic ratio of polymeric micelles on their endocytosis pathways into cells.
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DOI：
10.1002/mabi.201300037
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发表时间：
2013-06
影响因子：
4.6
通讯作者：
Zhao Zhang;Qianqian Qu;Jinrong Li;Shaobing Zhou
中科院分区：
文献类型：
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作者：
Zhao Zhang;Qianqian Qu;Jinrong Li;Shaobing Zhou
将荧光探针异硫氰酸异黄绿素（FITC）偶联到两亲性单甲氧基聚乙二醇-聚己内酯（mPEG-PCL）共聚物上。FITC标记的mPEG-PCL共聚物通过溶剂蒸发法自组装成胶束。使用荧光显微镜检查NIH-3 T3成纤维细胞与胶束或游离FITC溶液孵育的细胞内化。通过荧光显微镜对人肝母细胞瘤细胞和人脐静脉内皮细胞的培养评价亲水/疏水比对内吞机制的影响，所述细胞分别与具有相同参数（包括胶束尺寸、形状和表面电荷）的胶束混合。
Fluorescein isothiocyanate (FITC), a fluorescent probe, is coupled to amphiphilic monomethoxy poly(ethylene glycol)-block-poly(ε-caprolactone) (mPEG-PCL) copolymers. FITC-labeled mPEG-PCL copolymers self-assemble into micelles through the solvent evaporation method. The cellular internalization is examined using fluorescence microscopy on incubation of NIH-3T3 fibroblasts with micelles or free FITC solution. The effect of the hydrophilic/hydrophobic ratio on the endocytosis mechanisms is evaluated by fluorescence microscopy on culturing of human hepatoblastoma cells and human umbilical vein endothelial cells, individually, mixed with the micelles holding the same parameters including micelle size, shape, and surface charges.