Expression of c-fos and BDNF mRNA in subregions of the prefrontal cortex of male and female rats after acute uncontrollable stress
Expression of c-fos and BDNF mRNA in subregions of the prefrontal cortex of male and female rats after acute uncontrollable stress
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DOI：
10.1016/j.brainres.2005.05.065
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发表时间：
2005-07-27
期刊：
影响因子：
2.9
通讯作者：
Maier, SF
中科院分区：
文献类型：
--
作者：
Bland, ST;Schmid, MJ;Maier, SF
女性表现出比男性更高的终生压力相关疾病患病率。这些疾病与前额皮质结构和功能的改变有关。在这里，我们研究了急性不可逃避应激（一种行为抑郁和创伤后应激障碍的动物模型）对雄性和循环雌性大鼠前额叶和额叶皮质区域血浆皮质酮（CORT）和c-fos mRNA和脑源性神经营养因子（BDNF） mRNA的影响。不可避免的压力包括100毫安的尾震，而无压力的对照组则保持在它们的笼子里。应激源终止后立即（0 min）或60 min处死大鼠。与对照组相比，在应激终止后0和60分钟，CORT水平均有所升高，女性在这两个时间点的升高幅度更大。c-fos mRNA表达在0 min时增加，但雄性的增加幅度大于发情期和发情前期的雌性。60 min时，男性c-fos mRNA水平低于0 min时，女性c-fos mRNA水平低于0 min时。在应激组中，女性前额叶区域的CORT和c-fos mRNA水平无相关性，但在男性的几个前额叶区域中观察到显著相关。雌性小鼠BDNF mRNA表达量高于雄性小鼠。不可逃避应激对雄性大鼠BDNF mRNA表达无影响，但对雌性大鼠BDNF mRNA表达无影响。这些结果揭示了不可避免的压力诱导基因表达的性别差异，这可能暗示了对压力相关疾病的易感性差异。(c) 2005 Elsevier B.V.版权所有
Women exhibit higher lifetime prevalences of stress-related disorders than men. These disorders have been associated with changes in prefrontal cortex structure and function. Here, we examine the effects of acute inescapable stress, an animal model of behavioral depression and post-traumatic stress disorder, on plasma corticosterone (CORT) and on c-fos mRNA and brain-derived neurotrophic factor (BDNF) mRNA in regions of the prefrontal and frontal cortex in male and cycling female rats. Inescapable stress consisted of 100 1 mA tailshocks, and no-stress controls remained in their home cages. Rats were sacrificed immediately (0 min) or 60 min after termination of the stressor. CORT levels were increased at both 0 and 60 min post-stress termination relative to controls, and the increase was greater in females at both time points. c-fos mRNA expression increased at 0 min in prefrontal cortical regions, but this increase was greater in males than estrus and proestrus females. At 60 min, c-fos mRNA levels were lower than at 0 thin in males but not females. No correlations between CORT and c-fos mRNA levels in prefrontal regions were observed in females in the stress groups, but significant correlations were observed in males in several prefrontal regions. BDNF mRNA expression was greater in control females than control males. Inescapable stress increased BDNF mRNA expression at 0 but not 60 min in males, but there was no effect of inescapable stress on BDNF mRNA in females. These results reveal sex differences in inescapable stress-induced gene expression that may have implications for differences in vulnerability to stress-related disorders. (c) 2005 Elsevier B.V. All rights reserved.