Acetylcholinesterase biosensor based on gold nanoparticles and cysteamine self assembled monolayer for determination of monocrotophos.
Acetylcholinesterase biosensor based on gold nanoparticles and cysteamine self assembled monolayer for determination of monocrotophos.
复制标题
基于金纳米粒子和半胱胺自组装单层的乙酰胆碱酯酶生物传感器用于测定久效磷。
DOI：
10.1166/jnn.2009.se24
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发表时间：
2009
影响因子：
--
通讯作者：
Aidong Zhang
中科院分区：
文献类型：
--
作者：
Dan Du;Wenjuan Chen;Jie Cai;Jianming Zhang;Haiyang Tu;Aidong Zhang
本文提出了一种简单的固定乙酰胆碱酯酶（AChE）于半胱胺-纳米金（GNP）组装玻碳电极上的方法，从而建立了一种灵敏、快速、稳定的电流型生物传感器，用于久效磷的定量测定。通过循环伏安法、电化学阻抗谱和接触角等测试手段对制备过程进行了表征。GNP的存在不仅增加了有效表面积，为酶的结合提供了足够的位点，而且促进了电子转移反应，催化了硫代胆碱的电氧化，从而提高了检测灵敏度。由于固定化乙酰胆碱酯酶的伏安信号显著降低，建立了测定久效磷的简单方法。在0.5 ~ 10 ng·mL ~（-1）和10 ~ 600 ng·mL ~（-1）两个浓度范围内，久效磷的抑制作用与其浓度成正比，检测限低于0.3 ng·mL ~（-1）。所构建的生物传感器具有良好的精密度和重现性、可接受的稳定性和准确度、快速响应和低检测限等突出特点和性能，在有毒化合物的检测中具有潜在的应用前景。
In this paper, a simple method for immobilization of acetylcholinesterase (AChE) on cysteamine assembled glassy carbon electrode coupled with gold nanoparticles (GNPs) was proposed and thus a sensitive, fast and stable amperometric biosensor for quantitative determination of monocrotophos was developed. The fabrication procedure was characterized by cyclic voltammetry, electrochemical impedance spectroscopy and contact angles. The presence of GNPs not only led to an increased effective surface to provide a sufficient amount of sites for binding enzyme, but also promoted electron transfer reactions and catalyzed the electro-oxidation of thiocholine, thus amplifying the detection sensitivity. Due to the notable decrease in voltammetric signal of the immobilized AChE, a simple method for determination of monocrotophos was established. The inhibition of monocrotophos was proportional to its concentration in two ranges, from 0.5 to 10 ng mL(-1) and from 10 to 600 ng mL(-1), with a detection limit lower than 0.3 ng mL(-1). The constructed biosensor processing prominent characteristics and performance such as good precision and reproducibility, acceptable stability and accuracy, fast response and low detection limit has potential application in detection of toxic compounds.