Tandem array-based expression screens identify host mRNA targets of virus-encoded microRNAs.
Tandem array-based expression screens identify host mRNA targets of virus-encoded microRNAs.
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DOI：
10.1038/ng.266
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发表时间：
2009-01
期刊：
影响因子：
30.8
通讯作者：
Ganem, Don
中科院分区：
文献类型：
--
作者：
Ziegelbauer, Joseph M.;Sullivan, Christopher S.;Ganem, Don
MicroRNAs (miRNAs) are short non-coding RNAs of cellular and viral origin that post-transcriptionally regulate gene expression through imperfect base pairing to their mRNA targets. Because the recognition sequences of miRNAs for their targets are short and may be discontinuous, bioinformatic prediction of targets is difficult. Here we present an approach to the experimental identification of the mRNA targets of miRNAs encoded by the Kaposi's sarcoma-associated herpesvirus (KSHV). KSHV encodes 17 miRNAs, derived from 12 pre-miRNAs expressed from a single locus during viral latency. Our approach is based upon multiple screens that examine small changes in transcript abundance under different conditions of miRNA expression or inhibition, followed by searching the identified transcripts for seed sequence matches. This strategy led to the identification of the Bcl2-associated factor BCLAF1 as a target for miR-K5, and further analysis revealed that several other KSHV miRNAs also target this gene product. Our results support that this type of expression profiling provides a potentially general approach to the identification of miRNA targets.
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影响因子：
5.4
作者：
Skalsky, Rebecca L.;Samols, Mark A.;Renne, Rolf
通讯作者：
Renne, Rolf
影响因子：
64.8
作者：
Baek, Daehyun;Villen, Judit;Shin, Chanseok;Camargo, Fernando D.;Gygi, Steven P.;Bartel, David P.
通讯作者：
Bartel, David P.
DOI：
10.1126/science.1139253
发表时间：
2007-04-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Rodriguez A;Vigorito E;Clare S;Warren MV;Couttet P;Soond DR;van Dongen S;Grocock RJ;Das PP;Miska EA;Vetrie D;Okkenhaug K;Enright AJ;Dougan G;Turner M;Bradley A
通讯作者：
Bradley A
影响因子：
56.9
作者：
Jopling, CL;Yi, MK;Sarnow, P
通讯作者：
Sarnow, P
影响因子：
4.5
作者：
Grundhoff, A;Sullivan, CS;Ganem, D
通讯作者：
Ganem, D