GADD45β, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens.
GADD45β, an anti-tumor gene, inhibits avian leukosis virus subgroup J replication in chickens.
复制标题
抗肿瘤基因 GADD45 beta 抑制鸡体内禽白血病病毒 J 亚型复制
DOI：
10.18632/oncotarget.12027
复制
发表时间：
2016-10-18
期刊：
影响因子：
--
通讯作者：
Xie Q
中科院分区：
文献类型：
--
作者：
Zhang X;Yan Z;Li X;Lin W;Dai Z;Yan Y;Lu P;Chen W;Zhang H;Chen F;Ma J;Xie Q
禽白血病病毒J亚群（ALV-J）是一种逆转录病毒，可引起鸡瘤变、肝肿大、免疫抑制和生产性能下降。ALV-J的致瘤性和致病机制一直是研究的热点。为了探索促进鸡抗ALV-J感染的抗肿瘤基因，我们培育了ALV-J耐药和易感鸡（F3代）。对ALV-J抗性和易感鸡肝组织进行rna测序（RNA-Seq），鉴定出216个差异表达基因；其中88个基因在ALV-J抗性鸡中上调（与易感鸡相比）。我们筛选了在ALV-J抗性鸡中显著上调的基因（P < 0.01），基于它们参与生物信号通路。功能分析显示，过表达GADD45β可抑制ALV-J的复制。GADD45β可增强对ALV-J感染的防御，可作为ALV-J感染的分子标记物。
Avian leukosis virus subgroup J (ALV-J) is a retroviruses that induces neoplasia, hepatomegaly, immunosuppression and poor performance in chickens. The tumorigenic and pathogenic mechanisms of ALV-J remain a hot topic. To explore anti-tumor genes that promote resistance to ALV-J infection in chickens, we bred ALV-J resistant and susceptible chickens (F3 generation). RNA-sequencing (RNA-Seq) of liver tissue from the ALV-J resistant and susceptible chickens identified 216 differentially expressed genes; 88 of those genes were up-regulated in the ALV-J resistant chickens (compared to the susceptible ones). We screened for significantly up-regulated genes (P < 0.01) of interest in the ALV-J resistant chickens, based on their involvement in biological signaling pathways. Functional analyses showed that overexpression of GADD45β inhibited ALV-J replication. GADD45β could enhance defense against ALV-J infection and may be used as a molecular marker to identify ALV-J infections.