Changes in relaxin production by the theca during the preovulatory period of the pig.
Changes in relaxin production by the theca during the preovulatory period of the pig.
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猪排卵前期间卵泡膜松弛素产生的变化。
DOI：
10.1530/jrf.0.0690677
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
D. G. Porter
中科院分区：
文献类型：
--
作者：
G. Evans;D. Wathes;G. J. King;D. Armstrong;D. G. Porter
从未处理的青春期前母猪的排卵前卵泡中分离卵泡膜和颗粒层（A组），1000 i.u. PMSG（B组）、PMSG后84 h处死（C组）或PMSG + 500 i.u. D组：72 h后给予hCG。将单个卵泡的组织单独培养（C）、与FSH（F）或与LH（L）培养24 h，用放射免疫法测定培养液中松弛素的含量。在所有颗粒细胞培养物和未处理后备母猪的卵泡膜培养物中，松弛素样物质的浓度接近检测限。然而，所有19只处理后备母猪的卵泡膜培养物均产生松弛素。平均值+/- s.e.m.在AC、BC、CC和DC组中的浓度（pg/卵泡）分别为26 X 5 +/-3 X 04、93 X 1 +/-4 X 6、138 +/-16 X 4和285 X 6 +/-54 X 1。因此，PMSG刺激松弛素的产生（P小于0 × 0.05），体内hCG处理导致进一步显著增加（P小于0 × 0.05）。促性腺激素体外处理对A、C和D组无影响，但在B组后备母猪中，LH使松弛素水平显著升高（P <0 × 05）。这些研究表明卵泡膜是猪排卵前卵泡松弛素的主要来源。排卵前松弛素的增加表明松弛素可能参与卵泡的生长或破裂。
Theca and granulosa layers were isolated from the preovulatory follicles of prepubertal gilts which were untreated (Group A), killed 72 h after 1000 i.u. PMSG (Group B), killed 84 h after PMSG (Group C), or killed 84 h after PMSG + 500 i.u. hCG given at 72 h (Group D). The tissues from individual follicles were cultured for 24 h alone (C), with FSH (F) or with LH (L), and the content of immunoreactive relaxin in the culture media was measured by RIA. Concentrations of relaxin-like material were close to the limit of detection of the assay in all granulosa cell cultures, and in the thecal cultures from the untreated gilts. However, thecal cultures from all 19 treated gilts produced relaxin. The mean +/- s.e.m. concentrations (pg/follicle) in Groups AC, BC, CC and DC were 26 X 5 +/- 3 X 04, 93 X 1 +/- 4 X 6, 138 +/- 16 X 4 and 285 X 6 +/- 54 X 1 respectively. Therefore relaxin production was stimulated by PMSG (P less than 0 X 05), with hCG treatment in vivo leading to a further significant increase (P less than 0 X 05). In-vitro treatment with gonadotrophins had no effect in Groups A, C and D, but in Group-B gilts LH produced a significant (P less than 0 X 05) rise in relaxin levels. These studies indicate that the theca is the principal source of relaxin in the porcine preovulatory follicle. The increased production before ovulation suggests that relaxin may be involved in follicular growth or rupture.