Evidence of SV40 infections in hospitalized children
Evidence of SV40 infections in hospitalized children
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DOI：
10.1016/s0046-8177(99)90173-9
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发表时间：
1999-12-01
期刊：
影响因子：
3.3
通讯作者：
Adam, E
中科院分区：
文献类型：
--
作者：
Butel, JS;Jafar, S;Adam, E
已知猿猴病毒40(SV40)在1955年至1963年期间使用过受污染的脊髓灰质炎病毒疫苗。越来越多的报告描述了SV40 DNA在人类肿瘤和正常组织中的存在，尽管人类感染SV40的意义尚不清楚。我们调查了未经选择的住院儿童是否有SV40感染的证据，以及是否存在明显的临床相关性。血清样本用特异性空斑减少试验检测SV40中和抗体；在检测的337个样本中，有20个样本(5.9%)具有SV40抗体。血清阳性率随年龄增长而增加，并与肾移植显著相关(6/15[40%]阳性，P<；.001)。许多抗体阳性的患者免疫系统受损。对档案组织标本的分子分析(聚合酶链式反应和DNA序列分析)证实4例抗体阳性患者中存在SV40DNA。这项研究使用了两种独立的检测方法，显示了1980年后出生的儿童中存在SV40感染。我们得出结论，SV40会引起人类的自然感染。版权所有(C)1999，由W.B.Saunders公司提供。
Simian virus 40 (SV40) is known to have contaminated poliovirus vaccines used between 1955 and 1963. Accumulating reports have described the presence of SV40 DNA in human tumors and normal tissues, although the significance of human infections by SV40 is unknown. We investigated whether unselected hospitalized children had evidence of SV40 infections and whether any clinical correlations were apparent. Serum samples were examined for SV40 neutralizing antibody using a specific plaque reduction test; of 337 samples tested, 20 (5.9%) had antibody to SV40. Seropositivity increased with age and was significantly associated with kidney transplants (6 of 15 [40%] positive, P < .001). Many of the antibody-positive patients had impaired immune systems. Molecular assays (polymerase chain reaction and DNA sequence analysis) on archival tissue specimens confirmed the presence of SV40 DNA in 4 of the antibody-positive patients. This study, using 2 independent assays, shows the presence of SV40 infections in children born after 1980. We conclude that SV40 causes natural infections in humans. Copyright (C) 1999 by W.B. Saunders Company.