Experimental model of lacunar infarction in the gyrencephalic brain of the miniature pig - Neurological assessment and histological, immunohistochemical, and physiological evaluation of dynamic corticospinal tract deformation
Experimental model of lacunar infarction in the gyrencephalic brain of the miniature pig - Neurological assessment and histological, immunohistochemical, and physiological evaluation of dynamic corticospinal tract deformation
复制标题
DOI：
10.1161/strokeaha.107.489906
复制
发表时间：
2008-01-01
期刊：
影响因子：
8.3
通讯作者：
Saito, Nobuhito
中科院分区：
文献类型：
--
作者：
Tanaka, Yukitaka;Imai, Hideaki;Saito, Nobuhito
背景与目的腔隙性脑梗塞占缺血性卒中的25%，但其病理特征尚未得到系统研究。建立一种新的小型猪腔隙性脑梗塞模型，观察皮质脊髓束从急性期到慢性期的病理生理变化。方法35只小型猪接受经颅手术治疗永久性脉络膜前动脉闭塞。动物恢复时间分别为24小时(n=7)、2(n=5)、3(n=2)、4(n=2)、6(n=1)、7(n=7)、8(n=2)和9天(n=1)、2周(n=2)、4周(n=3)和4周以上(n=3)。进行神经学、电生理学、组织学和核磁共振检查。另外7只小型猪在短暂性脉络膜前动脉闭塞时进行肌肉运动诱发电位检测和皮质脊髓束功能评估。结果：该方法诱导286+/-153 mm(3)(平均+/-SD)内囊梗塞的成功率为91.4%。运动诱发电位显示，在脉络膜前动脉阻断6-15分钟后，内囊内存在半暗带组织。永久性脉络膜前动脉闭塞组和假手术组神经功能总评分分别为15.0(95%CI，13.5~16.4)和3.4(0.3~6.4)(P<；0.001，满分2 5)，运动功能障碍评分分别为3.4(95%CI，2.9~4.0)和0.0(CI，0.0~0)(P<；0.001，最高评分9)。组织学显示内囊病变从急性期到慢性期逐渐扩大。结论：这种新的腔隙性脑梗塞模型导致皮质下白质内可复制的脑梗塞，有可测量的功能缺陷和急性半暗带组织的证据。
Background and Purpose-Lacunar infarction accounts for 25% of ischemic strokes, but the pathological characteristics have not been investigated systematically. A new experimental model of lacunar infarction in the miniature pig was developed to investigate the pathophysiological changes in the corticospinal tract from the acute to chronic phases.Methods-Thirty-five miniature pigs underwent transcranial surgery for permanent anterior choroidal artery occlusion. Animals recovered for 24 hours (n = 7), 2 (n = 5), 3 (n = 2), 4 (n = 2), 6 (n = 1), 7 (n = 7), 8 (n = 2), and 9 days (n = 1), 2 weeks (n = 2), 4 weeks (n = 3), and more than 4 weeks (n = 3). Neurology, electrophysiology, histology, and MRI were performed. Seven additional miniature pigs underwent transient anterior choroidal artery occlusion to study muscle motor-evoked potentials and evaluate corticospinal tract function during transient anterior choroidal artery occlusion.Results-The protocol had a 91.4% success rate in induction of internal capsule infarction 286 +/- 153 mm(3) ( mean +/- SD). Motor-evoked potentials revealed the presence of penumbral tissue in the internal capsule after 6 to 15 minutes anterior choroidal artery occlusion. Total neurological deficit scores of 15.0 (95% CI, 13.5 to 16.4) and 3.4 (0.3 to 6.4) were recorded for permanent anterior choroidal artery occlusion and sham groups, respectively (P < 0.001, maximum score 25) with motor deficit scores of 3.4 (95% CI, 2.9 to 4.0) and 0.0 (CI, 0.0 to 0.0), respectively (P < 0.001, maximum score 9). Histology revealed that the internal capsule lesion expands gradually from acute to chronic phases.Conclusions-This new model of lacunar infarction induces a reproducible infarct in subcortical white matter with a measurable functional deficit and evidence of penumbral tissue acutely.