Identification and characterization of two fatty acid elongases in Lipomyces starkeyi
Identification and characterization of two fatty acid elongases in Lipomyces starkeyi
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DOI：
10.1007/s00253-020-10401-9
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发表时间：
2020-03-01
影响因子：
5
通讯作者：
Yaoi, Katsuro
中科院分区：
文献类型：
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作者：
Matsuzawa, Tomohiko;Kamisaka, Yasushi;Yaoi, Katsuro
产油酵母斯达氏油脂酵母是一种潜在的具有成本效益的微生物油脂生产源。脂肪酸延伸酶在长链脂肪酸的合成中起着重要的作用。本研究对两个编码L. starkeyi、Ls 1和Ls 2进行了鉴定和表征。这些基因在酿酒酵母中的异源表达表明，LsElo 1参与了具有24个碳原子的饱和长链脂肪酸（C24：0）的生产，LsElo 2参与了C16脂肪酸向C18脂肪酸的转化。此外，LsElo 1和LsElo 2都能够延长多不饱和脂肪酸。LsElo 1将亚油酸（C18：2）延长为二十碳二烯酸（C20：2），LsElo 2将α-亚麻酸（C18：3）延长为二十碳三烯酸（C20：3）。LsElo 2在L.斯塔克氏菌引起C16脂肪酸如棕榈酸和棕榈油酸的减少，以及C18脂肪酸如油酸和亚油酸的积累。我们的研究结果有可能有助于脂肪酸组成的重塑和产油酵母中多不饱和长链脂肪酸的生产。
The oleaginous yeast Lipomyces starkeyi is a potential cost-effective source for the production of microbial lipids. Fatty acid elongases have vital roles in the syntheses of long-chain fatty acids. In this study, two genes encoding fatty acid elongases of L. starkeyi, LsELO1, and LsELO2 were identified and characterized. Heterologous expression of these genes in Saccharomyces cerevisiae revealed that LsElo1 is involved in the production of saturated long-chain fatty acids with 24 carbon atoms (C24:0) and that LsElo2 is involved in the conversion of C16 fatty acids to C18 fatty acids. In addition, both LsElo1 and LsElo2 were able to elongate polyunsaturated fatty acids. LsElo1 elongated linoleic acid (C18:2) to eicosadienoic acid (C20:2), and LsElo2 elongated alpha-linolenic acid (C18:3) to eicosatrienoic acid (C20:3). Overexpression of LsElo2 in L. starkeyi caused a reduction in C16 fatty acids, such as palmitic and palmitoleic acids, and an accumulation of C18 fatty acids such as oleic and linoleic acids. Our findings have the potential to contribute to the remodeling of fatty acid composition and the production of polyunsaturated long-chain fatty acids in oleaginous yeasts.