INJECTION OF DIGITALLY SYNTHESIZED SYNAPTIC CONDUCTANCE TRANSIENTS TO MEASURE THE INTEGRATIVE PROPERTIES OF NEURONS
INJECTION OF DIGITALLY SYNTHESIZED SYNAPTIC CONDUCTANCE TRANSIENTS TO MEASURE THE INTEGRATIVE PROPERTIES OF NEURONS
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DOI：
10.1016/0165-0270(93)90119-c
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发表时间：
1993-09-01
影响因子：
3
通讯作者：
KAWAI, N
中科院分区：
文献类型：
--
作者：
ROBINSON, HPC;KAWAI, N
A novel technique was developed for injecting a time-varying conductance into a neuron, to allow quantitative measurement of the processing of synaptic inputs. In current-clamp recording mode, the membrane potential was sampled continuously and used to calculate and update the level of injected current within 60 mus, using a real-time computer, so as to mimic the electrical effect of a given conductance transient. Cellular responses to synthetic conductance transients modelled on the fast (non-N-methyl-D-aspartate) phase of the glutamatergic postsynaptic potential were measured in cultured rat hippocampal neurons.
