Single-cell measurements of human neutrophil activation using electrorotation
Single-cell measurements of human neutrophil activation using electrorotation
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DOI：
10.1021/ac980070c
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发表时间：
1998-07-01
影响因子：
7.4
通讯作者：
Cooper, JM
中科院分区：
文献类型：
--
作者：
Griffith, AW;Cooper, JM
我们已经使用了细胞电旋转的技术内的一个微加工的平面金电极阵列作为一种手段，检测一个单一的人类中性粒细胞的物理性质的变化，当被激活的趋化因子，佛波醇肉豆蔻酸酯乙酸酯（PIMA）。使用双壳模型来代表细胞及其细胞核，分析结果提供了细胞激活过程中发生的生物物理参数变化的指示。本研究中使用的方法在药物筛选的单细胞测定的发展中具有潜在的应用，作为快速确定药物作用的手段。
We have used the technique of cell electrorotation within a microfabricated planar gold electrode array as a means of detecting changes in the physical properties of a single human neutrophil, when activated by the chemotactic factor, phorbol myristate acetate (PIMA). The results, which have been analyzed using a double-shell model to represent the cell and its nucleus, provide an indication of the changes in biophysical parameters that occur during cell activation. The methods used in this study have potential applications in the development of single-cell assays for pharmaceutical screening, as a means of determining rapidly the action of drugs.