The Spot 42 RNA: A regulatory small RNA with roles in the central metabolism
The Spot 42 RNA: A regulatory small RNA with roles in the central metabolism
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DOI：
10.1080/15476286.2015.1086867
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发表时间：
2015-10-03
期刊：
影响因子：
4.1
通讯作者：
Haugen, Peik
中科院分区：
文献类型：
--
作者：
Baekkedal, Cecilie;Haugen, Peik
Spot 42 RNA是由spf（spot 42）基因编码的109个核苷酸长（在大肠杆菌中）的非编码小调控RNA（sRNA）。spf在γ-变形菌中发现，并且Spot 42 RNA的大部分实验工作已经使用E.大肠杆菌，以及最近的杀鲑弧菌（Aliivibrio salmonicida）。在细胞中，Spot 42 RNA作为碳水化合物代谢和摄取的调节剂发挥重要作用，其表达被葡萄糖激活，并被cAMP-CRP复合物抑制。本文综述了Spot 42的分子生物学研究现状，介绍了Spot 42的自然分布、Spot 42家族特异性二级结构特征以及Spot 42与mRNA靶点结合的高度保守结构区域。
The Spot 42 RNA is a 109 nucleotide long (in Escherichia coli) noncoding small regulatory RNA (sRNA) encoded by the spf (spot fourty-two) gene. spf is found in gamma-proteobacteria and the majority of experimental work on Spot 42 RNA has been performed using E. coli, and recently Aliivibrio salmonicida. In the cell Spot 42 RNA plays essential roles as a regulator in carbohydrate metabolism and uptake, and its expression is activated by glucose, and inhibited by the cAMP-CRP complex. Here we summarize the current knowledge on Spot 42, and present the natural distribution of spf, show family-specific secondary structural features of Spot 42, and link highly conserved structural regions to mRNA target binding.