Effects of Episodic Alcohol Exposure on BMP2 Signaling During Tibia Fracture Healing.
Effects of Episodic Alcohol Exposure on BMP2 Signaling During Tibia Fracture Healing.
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DOI：
10.1097/bot.0000000000001160
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发表时间：
2018-06
影响因子：
2.3
通讯作者：
Callaci JJ
中科院分区：
文献类型：
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作者：
Bratton A;Eisenberg J;Vuchkovska A;Roper P;Callaci JJ
探索酒精如何影响BMP-2信号通路，该信号通路在骨折愈合过程中的骨和软骨形成中起关键作用。使用大鼠模型来证明酒精暴露对胫骨骨折愈合的不利影响。通过Western免疫测定和ELISA分析骨折后第3、7、14、21天骨痂中BMP-2通路的特定组分。胫骨骨折前酒精暴露表明下游BMP-2信号转导减弱。BMP-2拮抗剂Chordin可能是本研究中证实的BMP-2相关变化的中心成分。虽然酒精在所有时间点都影响BMP相关蛋白，但在我们的模型中，骨折后第14天似乎是酒精相关调节愈伤组织形成的关键时间点。这项研究可能为进一步研究提供科学依据，以确定外源性BMP-2应用于有酗酒史且发生长骨骨折的患者是否有益。
To explore how alcohol affects the BMP-2 signaling pathway, which is known to play a critical role in bone and cartilage formation during fracture healing. A rat model was used to demonstrate the detrimental effects of alcohol exposure on tibia fracture healing. Specific components of the BMP-2 pathway were analyzed in fracture callus at days 3, 7, 14, 21 post fracture via western immunoassays and ELISA. Alcohol exposure prior to tibia fracture demonstrated attenuation of downstream BMP-2 signaling. The BMP-2 antagonist, Chordin, may be the central component of the BMP-2 related changes demonstrated in this study. While alcohol affected BMP-related proteins at all time points, it appears day fourteen post fracture is a critical time point for alcohol-related modulation of callus formation in our model. This study may provide the scientific basis for further studies addressing whether the application of exogenous BMP-2 in patients with a history of alcohol abuse who sustain long bone fractures may or may not be of benefit.