Upregulation of FOXP4 in breast cancer promotes migration and invasion through facilitating EMT
Upregulation of FOXP4 in breast cancer promotes migration and invasion through facilitating EMT
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乳腺癌中 FOXP4 的上调通过促进 EMT 促进迁移和侵袭
DOI：
10.2147/cmar.s191641
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发表时间：
2019-01-01
影响因子：
3.3
通讯作者：
Zhang, Jin
中科院分区：
文献类型：
--
作者：
Ma, Tao;Zhang, Jin
背景资料：FOXP 4家族是一个重要的转录因子家族，在多种肿瘤的发生、发展中起着重要作用。材料与方法：本研究旨在探讨FOXP 4在乳腺癌中的作用。采用实时定量PCR和Western blot方法检测FOXP 4在BC组织标本和细胞系中的mRNA和蛋白表达。使用功能获得和丧失测定来探索FOXP 4在乳腺细胞系（包括MDA-MB-231和MCF-7细胞）中的详细作用。结果：FOXP 4能促进BC细胞的增殖、迁移和侵袭。此外，FOXP 4还促进上皮-间充质转化。使用ChIP、qChIP测定和双荧光素酶报告基因测定来检查Snail是否是FOXP 4的下游靶标。此外，Snail的过表达可以部分地挽救FOXP 4抑制对癌细胞迁移和侵袭的影响。结论：我们的研究结果表明，FOXP 4是一个关键的调节在BC。
Background: Family of forkhead box transcription factors has been found to play key roles in multiple types of cancer.Materials and methods: Our study is to decipher the effects of FOXP4 in human breast cancer (BC). Quantitative real-time polymerise chain reaction and Western blot analyses were performed to determine the mRNA and protein expressions of FOXP4 in BC tissue samples and cell lines. The gain and loss of function assay were used to explore the detailed roles of FOXP4 in breast cell lines, including MDA-MB-231 and MCF-7 cells. Its effect on BC growth, migration, and invasion were evaluated by colony formation assay, CCK-8 assay, wound-healing assay, and transwell invasion assay, respectively.Results: Our findings revealed that FOXP4 promotes cell proliferation, migration, as well as invasion of BC cells. Furthermore, FOXP4 also facilitates epithelial-mesenchymal transition. ChIP, qChIP assay, and dual luciferase reporter assay were used to examine whether Snail is a downstream target of FOXP4. Moreover, overexpression of Snail could partially rescue the effects of FOXP4 inhibition on cancer cell migration and invasion.Conclusion: Our findings revealed that FOXP4 is a critical regulator in BC.