Size effects of latex nanomaterials on lung inflammation in mice
Size effects of latex nanomaterials on lung inflammation in mice
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DOI：
10.1016/j.taap.2008.09.012
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发表时间：
2009-01-01
影响因子：
3.8
通讯作者：
Shimada, Akinori
中科院分区：
文献类型：
--
作者：
Inoue, Ken-ichiro;Takano, Hirohisa;Shimada, Akinori
纳米材料（nanomaterials）对敏感人群的影响尚未得到很好的阐明。一项研究检查了肺部暴露于（乳胶）纳米材料对小鼠中与脂多糖（LPS）或过敏原相关的肺部炎症的影响，特别是在其尺寸依赖性方面。在方案1中，将ICR雄性小鼠分为8个实验组，这些实验组经鼻内接受单次暴露于溶剂、三种尺寸（25、50和100 nm）的乳胶纳米材料（250 μ g/动物）、LPS（75 μ g/动物）或LPS加乳胶纳米材料。在方案2中，将ICR雄性小鼠分为8个实验组，这些实验组经鼻内重复暴露于溶媒、乳胶纳米材料（100 μ g/动物）、过敏原（卵清蛋白：OVA; 1 μ g/动物）或过敏原加乳胶纳米材料。在方案1中，所有尺寸的乳胶纳米材料加剧了LPS引起的肺部炎症，显示出促炎细胞因子的肺表达放大的总体趋势。此外，与单独的LIPS相比，LPS加纳米材料，特别是尺寸小于50 μ m的纳米材料，显著提高了纤维蛋白原、巨噬细胞趋化蛋白-1和角化细胞衍生的趋化因子和血管性血友病因子的循环水平。总体而言，较小的纳米材料的增强效果往往大于较大的纳米材料。在方案2中，所有尺寸的乳胶纳米材料均未显著增强过敏性哮喘的病理生理学，其特征在于嗜酸性粒细胞性肺部炎症和Ig产生，尽管小于50 nm的乳胶纳米材料显著诱导/增强嗜酸性粒细胞性肺部炎症。这些结果表明，乳胶纳米材料对两种类型（先天性和适应性免疫主导型）肺部炎症的影响不同。(C)2008年爱思唯尔公司All rights reserved.
Effects of nano-sized materials (nanomaterials) on sensitive population have not been well elucidated This. study examined the effects of pulmonary exposure to (latex) nanomaterials on lung inflammation related to lipopolysaccharide (LPS) or allergen in mice, especially in terms of their size-dependency. In protocol 1, ICR male mice were divided into 8 experimental groups that intratracheally received a single exposure to vehicle, latex nanomaterials (250 mu g/animal) with three sizes (25, 50, and 100 nm), LPS (75 mu g/animal), or LPS plus latex nanomaterials. In protocol 2, ICR male mice were divided into 8 experimental groups that intratracheally received repeated exposure to vehicle, latex nanomaterials (100 mu g/animal), allergen (ovalbumin: OVA; 1 mu g/animal), or allergen plus latex nanomaterials. In protocol 1, latex nanomaterials with all sizes exacerbated lung inflammation elicited by LPS, showing an overall trend of amplified lung expressions of proinflammatory cytokines. Furthermore, LPS plus nanomaterials, especially with size less than 50 turn, significantly elevated circulatory levels of fibrinogen, macrophage chemoattractant protein-1, and keratinocyte-derived chemoattractant, and von Willebrand factor as compared with LIPS alone The. enhancement tended overall to be greater with the smaller nanomaterials than with the larger ones. In protocol 2, latex nanomaterials with all sizes did not significantly enhance the pathophysiology of allergic asthma, characterized by eosinophilic lung inflammation and Igs production, although latex nanomaterials with less than 50 nm significantly induced/enhanced neutrophilic lung inflammation. These results suggest that latex nanomaterials differentially affect two types of (innate and adaptive immunity-dominant) lung inflammation. (C) 2008 Elsevier Inc. All rights reserved.