The protruding domain of the coat protein of Melon necrotic spot virus is involved in compatibility with and transmission by the fungal vector Olpidium bornovanus.
The protruding domain of the coat protein of Melon necrotic spot virus is involved in compatibility with and transmission by the fungal vector Olpidium bornovanus.
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DOI：
10.1016/j.virol.2010.03.020
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发表时间：
2010-06
期刊：
影响因子：
3.7
通讯作者：
T. Ohki;F. Akita;T. Mochizuki;Ayami Kanda;T. Sasaya;S. Tsuda
中科院分区：
文献类型：
--
作者：
T. Ohki;F. Akita;T. Mochizuki;Ayami Kanda;T. Sasaya;S. Tsuda
甜瓜坏死斑病毒（MNSV）的Chi和W株系分别由真菌载体Olpidium bornovanus的分离物Y1和NW1有效传播。通过对两株病毒外壳蛋白（CP）基因互换构建的嵌合病毒的分析，揭示了CP参与了MNSV与O. bornovanus和真菌传播的病毒。此外，基于Chi株的嵌合病毒与来自W株的CP的突出结构域的分析表明该结构域参与与游动孢子的相容性。两种MNSV株CP之间的三维结构的比较表明，这些氨基酸残基中的许多差异存在于病毒颗粒的表面上，这表明这些差异影响病毒对真菌载体的识别。
The Chi and W strains of Melon necrotic spot virus (MNSV) are efficiently transmitted by isolates Y1 and NW1, respectively, of the fungal vector Olpidium bornovanus. Analysis of chimeric viruses constructed by switching the coat protein (CP) gene between the two strains unveiled the involvement of the CP in the attachment of MNSV to zoospores of a compatible isolate of O. bornovanus and in the fungal transmission of the virus. Furthermore, analysis of the chimeric virus based on the Chi strain with the protruding domain of the CP from strain W suggested the involvement of the domain in compatibility with zoospore. Comparison of the three-dimensional structures between the CP of the two MNSV strains showed that many of the differences in these amino acid residues are present on the surface of the virus particles, suggesting that these affects the recognition of fungal vectors by the virus.