Spectroscopic and morphological studies on interaction between gold nanoparticle and liposome constructed with phosphatidylcholine
Spectroscopic and morphological studies on interaction between gold nanoparticle and liposome constructed with phosphatidylcholine
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DOI：
10.1088/1757-899x/76/1/012001
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发表时间：
2015-03
期刊：
影响因子：
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通讯作者：
C. Tsukada;T. Tsuji;K. Matsuo;T. Nomoto;G. Kutluk;M. Sawada;S. Ogawa;T. Yoshida;S. Yagi
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文献类型：
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作者：
C. Tsukada;T. Tsuji;K. Matsuo;T. Nomoto;G. Kutluk;M. Sawada;S. Ogawa;T. Yoshida;S. Yagi
分别采用溶液等离子体法和挤压法制备了金纳米粒子（Au NPs）胶体溶液和磷脂酰胆碱（PC）脂质体水溶液。当Au NPs胶体溶液和PC脂质体水溶液混合时，考虑TEM图像，我们认为Au NPs首先被PC分子覆盖，这无助于形成PC脂质体，随后被PC覆盖的Au NPs吸附在PC脂质体表面。由于PC/Au层的NEXAFS谱中N、O和P K边的各峰强度均比PC多层膜的低，我们认为PC分子吸附在Au层表面的N-CH 3的甲基和P-O、P=O、C-O和C=O键的氧原子上.此外，PC覆盖的Au NPs似乎通过Au NPs上PC的疏水基团彼此聚集。
The gold nanoparticles (Au NPs) colloidal solution and the phosphatidylcholine (PC) liposome aqueous solution are fabricated by the solution plasma method and the extrusion procedure, respectively. When the Au NPs colloidal solution and the PC liposome aqueous solution are mixed, considering the TEM image, we think that the Au NPs firstly are covered with the PC molecules, which do not contribute to form the PC liposome, and subsequently the Au NPs covered with the PC adsorb on the PC liposome surface. We propose that the PC molecule adsorbs on the Au sheet surface at the methyl group of N-CH3 and the oxygen atoms of P-O, P=O, C-O and C=O bonds, because each peak intensity of N, O and P K-edges NEXAFS spectra for the PC/Au sheet is reduced in comparison with that for the PC multilayer. Furthermore, the Au NPs covered with PC seem to be aggregated each other through the hydrophobic groups of PC on Au NPs.