Evidence that a chlorophyll a' dimer constitutes the photochemical reaction centre 1 (P700) in photosynthetic apparatus
Evidence that a chlorophyll a' dimer constitutes the photochemical reaction centre 1 (P700) in photosynthetic apparatus
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叶绿素a二聚体构成光合装置中光化学反应中心1（P700）的证据
DOI：
10.1016/0014-5793(85)80019-3
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发表时间：
1985
期刊：
影响因子：
3.5
通讯作者：
N. Murata
中科院分区：
文献类型：
--
作者：
Tadashi Watanabe;Masami Kobayashi;A. Hongu;M. Nakazato;T. Hiyama;N. Murata
通过高效液相色谱法（HPLC）分析了菠菜叶绿体及其在光化学反应中心1（P700）或反应中心2（P680）中富集的各种组分的色素组成。结果清楚地建立了叶绿素（Chl）a '/P700 = 2的化学计量比，其中Chla'代表Chla的C10差向异构体。这是首次证明Chla'与体内任何功能位点的关联。它进一步表明，在体外制备的Chla'二聚体再现相当好的氧化还原和光谱行为的P700。这些发现提供了强有力的证据表明Chladimer构成了P700。
Spinach chloroplasts and their various fractions enriched in either photochemical reaction centre 1 (P700) or reaction centre 2 (P680) have been analyzed for pigment composition by high‐performance liquid chromatography (HPLC). The results clearly establish a stoichiometry of chlorophyll (Chl)a'/P700 = 2, where Chla' represents the C 10 epimer of Chla. This is the first demonstration of the association of Chla'with any functional site in vivo. It is further shown that a Chla' dimer prepared in vitro reproduces fairly well the redox and spectroscopic behaviour of P700. These findings provide strong evidence that a Chladimer constitutes P700.