Temperature regulates fatty acid desaturases at a transcriptional level and modulates the fatty acid profile in the Antarctic microalga Chlamydomonas sp ICE-L
Temperature regulates fatty acid desaturases at a transcriptional level and modulates the fatty acid profile in the Antarctic microalga Chlamydomonas sp ICE-L
复制标题
温度在转录水平上调节脂肪酸去饱和酶，并调节南极微藻衣藻中的脂肪酸谱。
DOI：
10.1016/j.biortech.2013.01.142
复制
发表时间：
2013-04-01
影响因子：
11.4
通讯作者：
Miao, Jinlai
中科院分区：
文献类型：
--
作者：
An, Meiling;Mou, Shanli;Miao, Jinlai
衣原体ICE-L能在南极的极端环境中生长，是一种重要的生物质生产者。获得了衣原体ICE-L的FAD基因，序列比对表明，这些基因与已知的具有保守组氨酸基序的FAD同源。在这项研究中，我们分析了五个FAD和FA组成在不同温度下的转录。结果表明，在0 ℃时，Delta 9 CiFAD、omega 3CiFAD 1和omega 3CiFAD 2的表达明显上调，而Delta 6CiFAD的表达随着温度的升高而增强。同时，分析的FA组成表明，多不饱和脂肪酸占优势的组成，占75%以上的总脂肪酸在衣原体。ICE-L此外，多不饱和脂肪酸显着增加，在0和5 ℃，这可能是由于较高的比例C18：3和C20：3。此外，PUFA在15 ℃时显著降低，而SFA显著增加。(C)2013爱思唯尔有限公司保留所有权利。
Chlamydomonas sp. ICE-L which can thrive in extreme environments of the Antarctic is a major biomass producer. The FAD genes in Chlamydomonas sp. ICE-L were obtained and sequence alignment showed that these genes are homologous to known FADs with conserved histidine motifs. In this study, we analyzed the transcription of five FADs and FA compositions at different temperatures. The results showed that the expressions of Delta 9CiFAD, omega 3CiFAD1 and omega 3CiFAD2 were apparently up-regulated at 0 degrees C, however, the up-regulation of Delta 6CiFAD intensified with rising temperature. Meanwhile, analysis of the FA compositions showed that PUFAs were dominant compositions, accounting for more than 75% TFA in Chlamydomonas sp. ICE-L Furthermore, PUFAs were significantly increased at 0 and 5 degrees C, which may be attributed to higher proportions of C18:3 and C20:3. Moreover, PUFAs were significantly decreased at 15 degrees C whereas SFAs were significantly increased. (C) 2013 Elsevier Ltd. All rights reserved.