Y-Maze memory task in zebrafish (Danio rerio): The role of glutamatergic and cholinergic systems on the acquisition and consolidation periods
Y-Maze memory task in zebrafish (Danio rerio): The role of glutamatergic and cholinergic systems on the acquisition and consolidation periods
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DOI：
10.1016/j.nlm.2012.09.008
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发表时间：
2012-11-01
影响因子：
2.7
通讯作者：
Bonan, Carla D.
中科院分区：
文献类型：
--
作者：
Cognato, Giana de P.;Bortolotto, Josiane W.;Bonan, Carla D.
在过去的二十年里，人们对斑马鱼行为特征的兴趣显着增加。然而，大多数可用的协议都使用了更长的训练期，并且基于强化/奖励或回避。Y迷宫记忆任务的优点是使用简单快速的训练课程，但它还没有在斑马鱼中建立。在这里，我们的特点是这项任务的斑马鱼，除了在收购和巩固记忆阶段的药理干预。结果表明，斑马鱼花更多的时间在新的手臂比其他武器的Y迷宫，无论是在响应新奇和空间记忆训练测试间隔（TTI）。我们还研究了NMDA受体拮抗剂MK-801（20 μ M）和胆碱能阻滞剂东莨菪碱（200 μ M）的训练前和训练后处理的多巴胺能和胆碱能系统的参与。TTI 1小时后，与对照组相比，预训练MK-801和东莨菪碱处理的鱼减少了对新臂的探索，其运动活性没有变化。MK-801治疗后的训练也损害了他们的Y-迷宫性能，而任何东莨菪碱治疗后的训练都没有影响新的手臂探索。总之，Y迷宫记忆任务可以可靠地用于斑马鱼，提供了一个新的，快速的，和偏好/回避独立的任务，在这个硬骨鱼的记忆研究。此外，我们的研究结果强调了斑马鱼记忆中的多巴胺能和胆碱能系统的意义。(C)2012 Elsevier Inc. All rights reserved.
The interest in the behavioral features of zebrafish has significantly increased over the past two decades. However, most available protocols have used longer training periods and have been based on reinforcement/reward or avoidance. The Y-Maze memory task has the advantage of using a simple and rapid training session, but it has not been established in zebrafish. Here, we have characterized this task for zebrafish, with the addition of pharmacological interventions in the acquisition and consolidation memory phases. The results show that zebrafish spend more time in the novel arm than in the other arms of the Y-Maze, both in response to novelty and spatial memory training-test intervals (TTIs). We have also studied the involvement of the glutamatergic and cholinergic systems with pre- and post-training treatments with the NMDA receptor antagonist MK-801 (20 mu M) and the cholinergic blocker scopolamine (200 mu M). After 1 h of TTI, pre-training MK-801 and scopolamine-treated fish reduced their exploration of the novel arm when compared to the control group, with no changes in their locomotor activity. Post-training of MK-801 treatment also impaired their Y-Maze performance, while post-training of any scopolamine treatment failed to affect novel arm exploration. In conclusion, the Y-Maze memory task can be reliably used for zebrafish, providing a new, rapid, and preference/avoidance independent task for the study of memory in this teleost. In addition, our results highlight the implication of the glutamatergic and cholinergic systems in the memory of zebrafish. (C) 2012 Elsevier Inc. All rights reserved.