3D diffractive imaging of nanoparticle ensembles using an x-ray laser
3D diffractive imaging of nanoparticle ensembles using an x-ray laser
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DOI：
10.1364/optica.410851
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发表时间：
2021-01-20
期刊：
影响因子：
10.4
通讯作者：
Chapman, Henry N.
中科院分区：
文献类型：
--
作者：
Ayyer, Kartik;Xavier, P. Lourdu;Chapman, Henry N.
在X射线自由电子激光（XFEL）的单粒子成像具有在室温下确定单个生物分子的结构和动力学的潜力。两个主要障碍阻碍了这种潜力的实现，即收集足够的高质量衍射图案和强大的计算纯化，以克服结构异质性。我们报告了使用金纳米颗粒测试样品打破这两个障碍，在欧洲XFEL记录了大约1000万个衍射图案，并对图案进行结构和方向分类，以获得四个样品中每个样品的3D重建优于3 nm分辨率。随着这些新的发展，集成了x射线源，快速成帧检测器，高效样品输送和数据分析算法的进步，我们照亮了亚纳米生物分子成像的道路。这里开发的方法也可以扩展到表征合奏，本质上是不同的，以获得其完整的结构景观。由The Optical Society根据Creative Commons Attribution 4.0 License条款发布。
Single particle imaging at x-ray free electron lasers (XFELs) has the potential to determine the structure and dynamics of single biomolecules at room temperature. Two major hurdles have prevented this potential from being reached, namely, the collection of sufficient high-quality diffraction patterns and robust computational purification to overcome structural heterogeneity. We report the breaking of both of these barriers using gold nanoparticle test samples, recording around 10 million diffraction patterns at the European XFEL and structurally and orientationally sorting the patterns to obtain better than 3-nm-resolution 3D reconstructions for each of four samples. With these new developments, integrating advancements in x-ray sources, fast-framing detectors, efficient sample delivery, and data analysis algorithms, we illuminate the path towards sub-nano meter biomolecular imaging. The methods developed here can also be extended to characterize ensembles that are inherently diverse to obtain their full structural landscape. Published by The Optical Society under the terms of the Creative Commons Attribution 4.0 License.