Geminin: A good prognostic factor in high-grade astrocytic brain tumors
Geminin: A good prognostic factor in high-grade astrocytic brain tumors
复制标题
DOI：
10.1002/cncr.22474
复制
发表时间：
2007-03-01
期刊：
影响因子：
6.2
通讯作者：
Kurisu, Kaoru
中科院分区：
文献类型：
--
作者：
Shrestha, Prabin;Saito, Taiichi;Kurisu, Kaoru
背景。 Geminin 是一种核蛋白，属于 DNA 复制抑制剂组。它通过阻止 Cdt1 将微型染色体维护蛋白加载到染色质上来抑制 DNA 复制，而这是 DNA 复制所需的。在这项研究中，作者研究了高级星形细胞肿瘤（包括间变性星形细胞瘤（AA）和多形性胶质母细胞瘤（GBM））中的geminin表达，以期在此基础上预测这些恶性脑肿瘤患者的临床结果。采用免疫组织化学方法检测51例高级别星形细胞瘤患者（19例AA和32例GBM）中geminin的表达。通过以中值作为构建 Kaplan-Meier 曲线的分界点对样本进行分类。使用Kaplan-Meier方法和Cox比例风险回归模型分析了这些恶性脑肿瘤中geminin表达与临床结果的关系。结果。 Geminin 在所有高级星形细胞瘤中均有表达（平均 Geminin 标记指数 [LI]，24.90%）。 Kaplan-Meier 曲线显示，geminin LI 较高的组 (>= 22.50%) 比 LI 较低的组 (< 22.50%；P = .0296) 预后更好。同样，Cox 回归分析显示，geminin 表达与高级别星形细胞瘤患者的生存率具有显着相关性 (P = .0278)，尤其是在早期阶段。结论。虽然它是 DNA 增殖的抑制剂，因此也是细胞周期抑制剂，但在所有恶性星形细胞肿瘤中都发现了 Geminin 的表达。 Geminin LI 是高级别星形细胞瘤患者预后的重要预测因素，较高的表达表明预后良好。
BACKGROUND. Geminin is a nuclear protein that belongs to the DNA replication inhibitor group. It inhibits DNA replication by preventing Cdt1 from loading minichromosome maintenance protein onto chromatin, as is required for DNA replication. For this study, the authors investigated geminin expression in high-grade astrocytic tumors, including anaplastic astrocytoma (AA) and glioblastoma multiforme (GBM), with a view to predicting clinical outcomes on this basis in patients with these malignant brain tumors.METHODS. Immunohistochemistry was used to detect geminin expression in 51 patients with high-grade astrocytic tumors (19 AA and 32 GBM). Samples were categorized by taking the median value as the cut-off point for constructing Kaplan-Meier curves. The relation of geminin expression to clinical outcome in these malignant brain tumors was analyzed by using the Kaplan-Meier method and a Cox proportional hazards regression model.RESULTS. Geminin was expressed in all high-grade astrocytomas (mean geminin labeling index [LI], 24.90%). Kaplan-Meier curves showed that the group with higher geminin LI (>= 22.50%) had a better prognosis than the group with lower LI (< 22.50%; P = .0296). Similarly, the Cox regression analysis showed that geminin expression has a significant correlation with survival in patients with high-grade astrocytoma (P = .0278), especially in an early stage.CONCLUSIONS. Although it is an inhibitor of DNA proliferation and, thus, is a cell cycle inhibitor, geminin expression was found in all malignant astrocytic tumors. The geminin LI was a significant predictive factor of outcomes in patients with high-grade astrocytoma, with higher expression indicating a good prognosis.