New human coronavirus, HCoV-NL63, associated with severe lower respiratory tract disease in Australia
New human coronavirus, HCoV-NL63, associated with severe lower respiratory tract disease in Australia
复制标题
DOI：
10.1002/jmv.20288
复制
发表时间：
2005-03-01
影响因子：
12.7
通讯作者：
Mackay, IM
中科院分区：
文献类型：
--
作者：
Arden, KE;Nissen, MD;Mackay, IM
一种新的人类冠状病毒 HCoV-NL63 最近与细支气管炎有关。当前的研究旨在使用针对 1a 和 1b 基因的巢式 RT-PCR 检测来检查回顾性保存的标本中是否存在 HCoV-NL63。研究人群由需要到昆士兰州医院就诊的急性呼吸道疾病患者组成。在代表 766 名患者的 840 份样本中，有 16 份的鼻咽抽吸物 (NPA) 中检测到了 HCoVNL63 (2%)。 HCoV-NL63 阳性个体最常被诊断患有下呼吸道 (LRT) 疾病 (81%)。临床诊断通常得到 HCoV-NL63 感染首次就诊时异常胸部 X 光检查 (56%) 以及呼吸窘迫 (50%)、喘息 (44%) 和呼吸困难 (25%) 的支持。所有 HCoV-NL63 呈阳性的患者均需入院。在 38% 的 HCoV-NL63 阳性标本中检测到第二种病原体。对基因 1b 的扩增子进行测序显示与病毒型毒株有超过 99% 的核苷酸同源性，而对基因 1a 的扩增子进行测序则允许对病毒株进行分组。研究表明，在一年中较凉爽的月份，HCoV-NL63 与澳大利亚医院环境中的严重 LRT 疾病有关。我们认为 HCoV-NL63 是一种与严重 LRT 疾病相关的儿童和成人的全球性季节性病原体。 (C) 2005Wiley-Liss，伊内。
A new human coronavirus, HCoV-NL63, was associated recently with bronchiolitis. The current study aimed to examine retrospectively stored specimens for the presence of HCoV-NL63 using nested RT-PCR assays targeting the 1a and 1b genes. The study population was composed of patients with acute respiratory disease warranting presentation to Queensland hospitals. HCoVNL63 was detected in the nasopharyngeal aspirates (NPA) of 16 of 840 specimens representing 766 patients (2%). HCoV-NL63 positive individuals were diagnosed most commonly with lower respiratory tract (LRT) disease (81%). The clinical diagnosis was commonly supported by an abnormal chest X-ray (56%) together with respiratory distress (50%), wheeze (44%), and r (a) over cap les (25%) on first presentation with HCoV-NL63 infection. All patients positive for HCoV-NL63 required admission to hospital. Among 38% of HCoV-NL63 positive specimens a second pathogen was detected. Sequencing of amplicon from gene 1b revealed more than 99% nucleotide homology with the viral type strains while sequencing amplicon from gene la permitted the grouping of viral strains. It was shown that HCoV-NL63 is associated with severe LRT disease in an Australian hospital setting during the cooler months of the year. We propose that HCoV-NL63 is a global and seasonal pathogen of both children and adults associated with severe LRT illness. (C) 2005Wiley-Liss,Ine.