Exploiting dynamic reaction cell inductively coupled plasma mass spectrometry (DRC-ICP-MS) for sequential determination of trace elements in blood using a dilute-and-shoot procedure
Exploiting dynamic reaction cell inductively coupled plasma mass spectrometry (DRC-ICP-MS) for sequential determination of trace elements in blood using a dilute-and-shoot procedure
复制标题
DOI：
10.1016/j.aca.2009.03.016
复制
发表时间：
2009-04-20
影响因子：
6.2
通讯作者：
Barbosa, Fernando, Jr.
中科院分区：
文献类型：
--
作者：
Batista, Bruno Lemos;Rodrigues, Jairo Lisboa;Barbosa, Fernando, Jr.
采用四极杆电感耦合等离子体质谱法（q-ICP-MS）和动态反应池（DRC-ICP-MS）连续测定血液中As、Cd、Co、Cr、Cu、Mn、Pb、Se、Tl、V、Zn。这种方法只需要100 μ L的血液。分析前，样品（100 μ L）在含有0.01% (v/v) Triton (R) X-100和0.5% （v/v）硝酸的溶液中1:50稀释。仅对Cr、Cu、V和Zn强制使用DRC。对于其他元件，设备可以在标准模式下操作（q-ICP-MS）。氨作为反应气体。采用羊底血检测Cr和V，用合成基质溶液（SMS）检测Zn和Cu，溶液稀释为1:50，加标为1 μ g L-1，选择最佳铵气流速，优化四极动态带通调谐参数（RPq）。方法对As-75、Cd-114、Co-59、Cr-51、Cu-63、Mn-55、Pb-208、Se-82、Tl-205、V-51和Zn-64的检出限（3 s）分别为14.0、3.0、11.0、7.0、280、9.0、3.0、264、0.7、6.0和800 ng L-1。通过对加拿大魁北克国家公共血液中心生产的血液标准物质进行分析，完成方法验证。(C) 2009 Elsevier B.V.版权所有
Inductively coupled plasma mass spectrometry with quadrupole (q-ICP-MS) and dynamic reaction cell (DRC-ICP-MS) were evaluated for sequential determination of As, Cd, Co, Cr, Cu, Mn, Pb, Se, Tl, V and Zn in blood. The method requires as little as 100 mu L of blood. Prior to analysis, samples (100 mu L) were diluted 1:50 in a solution containing 0.01% (v/v) Triton (R) X-100 and 0.5% (v/v) nitric acid. The use of the DRC was only mandatory for Cr, Cu, V and Zn. For the other elements the equipment may be operated in a standard mode (q-ICP-MS). Ammonia was used as reaction gas. Selection of best flow rate of ammonium gas and optimization of the quadrupole dynamic band-pass tuning parameter (RPq) were carried out, using a ovine base blood for Cr and V and a synthetic matrix solution (SMS) for Zn and Cu diluted 1:50 and spiked to contain 1 mu g L-1 of each element. Method detection limits (3 s) for As-75, Cd-114, Co-59, Cr-51, Cu-63 Mn-55, Pb-208, Se-82, Tl-205, V-51, and Zn-64 were 14.0, 3.0, 11.0, 7.0, 280, 9.0, 3.0, 264, 0.7, 6.0 and 800 ng L-1, respectively. Method validation was accomplished by the analysis of blood Reference Materials produced by the National cle Sant Publique du Quebec (Canada). (C) 2009 Elsevier B.V. All rights reserved.