DISORIENTED PATHFINDING BY PIONEER NEURON GROWTH CONES DEPRIVED OF FILOPODIA BY CYTOCHALASIN TREATMENT
DISORIENTED PATHFINDING BY PIONEER NEURON GROWTH CONES DEPRIVED OF FILOPODIA BY CYTOCHALASIN TREATMENT
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DOI：
10.1038/323712a0
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发表时间：
1986-10-23
期刊：
影响因子：
64.8
通讯作者：
TOROIANRAYMOND, A
中科院分区：
文献类型：
--
作者：
BENTLEY, D;TOROIANRAYMOND, A
发育神经生物学中的一个主要问题是发育中的神经细胞如何准确地扩展过程以建立与其靶细胞的连接。这个问题既涉及到生长锥引导线索的性质，也涉及到生长锥如何调查其环境中的线索，并通过改变其迁移方向作出反应的问题。正常情况下从神经元生长锥延伸的丝状伪足已被证明在体外影响生长锥转向1 - 4，并且已经提出它们在体内在检测和响应引导线索中起作用5 -10。这一假设可以在活体中得到验证，如果正常情况下具有丝状伪足的生长锥可以被诱导迁移。这对Ti 1神经元是第一个通过胚胎蝗虫肢芽延伸轴突的神经元11。在这里，我们报告了一个检查的迁移Ti 1先锋生长锥剥夺丝状伪足文化的代理人，破坏肌动蛋白微丝。在这些条件下，轴突继续延伸，但大部分生长锥高度迷失方向。我们的研究结果表明，Ti 1丝状伪足是不必要的轴突elongationin vivo，但它们是重要的正确定向生长锥转向。
A major question in developmental neurobiology is how developing nerve cells accurately extend processes to establish connections with their target cells. This problem involves both the nature of cues for growth cone guidance and also the question of how growth cones survey their environment for cues and respond by altering their direction of migration. The filopodia which normally extend from neuronal growth cones have been shown to affect growth cone steeringin vitro1–4and it has been proposed that they functionin vivoin the detection of and response to guidance cues5–10. This hypothesis could be testedin vivoif growth cones which normally have filopodia could be induced to migrate in their absence. The pair of Ti1 neurones are the first neurones to extend axons through the limb buds of embryonic grasshoppers11. We report here an examination of the migration of Ti1 pioneer growth cones deprived of filopodia by culture in agents which disrupt actin microfilaments. Under these conditions, axons continue to extend but a large percentage of growth cones are highly disoriented. Our results indicate that Ti1 filopodia are not necessary for axonal elongationin vivobut that they are important for correctly oriented growth cone steering.