Basophils are not the key antigen-presenting cells in allergic patients.
Basophils are not the key antigen-presenting cells in allergic patients.
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DOI：
10.1111/j.1398-9995.2012.02792.x
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发表时间：
2012-05
期刊：
影响因子：
12.4
通讯作者：
Niederberger V
中科院分区：
文献类型：
--
作者：
Eckl-Dorna J;Ellinger A;Blatt K;Ghanim V;Steiner I;Pavelka M;Valent P;Valenta R;Niederberger V
最近在小鼠模型中获得的数据引发了一场关于嗜碱性粒细胞是否是过敏反应中关键的抗原提呈细胞(APC)的争论。在这里，我们调查嗜碱性细胞是否对过敏患者的过敏原呈递和诱导T细胞增殖具有重要作用。从过敏性患者中提纯T细胞、嗜碱性粒细胞和去嗜碱性粒细胞的APC。建立了基于纯化的主要变应原的共培养系统，以利用增殖试验研究变应原特异性T细胞反应。只有与去嗜碱性粒细胞但不与嗜碱性粒细胞共培养的T细胞对变应原有增殖反应。即使在T细胞-嗜碱性粒细胞共培养中加入IL-3也不能诱导变应原特异性T细胞的增殖。我们的数据通过经典的体外增殖试验证明，在过敏患者对变应原的二次免疫反应中，嗜碱性粒细胞不是促进T细胞增殖的关键抗原提呈细胞。
Recent data obtained in mouse models have initiated a controversy whether basophils are the key antigen-presenting cells (APCs) in allergy. Here, we investigate whether basophils are of importance for the presentation of allergen and the induction of T cell proliferation in allergic patients. T cells, basophils, and APCs depleted of basophils were purified from allergic patients. Co-culture systems based on purified major allergens were established to study allergen-specific T cell responses using proliferation assays. Only co-cultures of T cells with APCs depleted of basophils but not with basophils proliferated in response to allergen. Even addition of IL-3 to T cell–basophil co-cultures failed to induce allergen-specific T cell proliferation. Our data demonstrate by classical in vitro proliferation assays that basophils are not key antigen-presenting cells that promote T cell proliferation in secondary immune responses to allergen in allergic patients.