Effects of selective RNA processing and stabilization enzymes on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002
Effects of selective RNA processing and stabilization enzymes on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002
复制标题
选择性 RNA 加工和稳定酶对聚球藻光合作用固碳的影响。
DOI：
10.3389/fmars.2023.1223060
复制
发表时间：
2023
影响因子：
--
通讯作者：
Ranran Huang
中科院分区：
文献类型：
--
作者：
Jinyu Chen;Daixi Liu;Yafei Wang;S. Wang;Ranran Huang
聚球藻是地球上最丰富的原核光合作用生物之一，在海洋碳固定和转化中起着关键作用。为了提高聚球藻的光合作用效率，考虑了一种转录后调控机制-选择性RNA处理和稳定(SRPS)。我们灭活了SRPS机制的执行者SRPS酶，以探索它们对聚球藻光合作用固碳效率的调节规律。结果表明，SRPS酶的失活主要影响生长速度或生长阶段。它显著改变了聚球藻(SOM)的光合作用放氧速率、色素含量、叶绿素荧光、碳和氮含量以及溶解有机物的组成和生物活性。失活SRPS酶导致细胞色素b6-f复合体大部分亚基的表达水平升高，而PSI、PSII、Rubisco和NDH的大部分亚基的表达水平下降。所有SRPS酶都参与了光合作用中碱性蛋白复合体的表达调控，如PSI、PSII、细胞色素b6-f复合体、ATP合成酶和Rubisco。结果表明，SRPS酶的失活对聚球藻的光合作用固碳有显著影响。PCC7002。
Synechococcus is one of the most abundant prokaryotic photosynthetic organisms on Earth and plays a key role in oceanic carbon fixation and transformation. To improve the photosynthetic efficiency of synechococcus, a post-transcriptional regulatory mechanism - Selective RNA Processing and Stabilization (SRPS) was considered. We inactivated the SRPS-enzymes, executor of the SRPS mechanism, to explore their regulation rule of photosynthetic carbon fixation efficiency in Synechococcus. The results showed that the inactivation of SRPS-enzymes mainly affected the growth rate or growth phase. It significantly alters the photosynthetic oxygen evolution rate, pigment content, chlorophyll fluorescence, carbon and nitrogen content, as well as the composition and biological activity of the dissolved organic matter derived from Synechococcus (SOM). Inactivating SRPS-enzymes results in an increase in the expression level of most subunits of the Cytochrome b6-f complex, while the expression levels of most subunits of PSI, PSII, RuBisCO, and NDH decrease. All SRPS-enzymes are involved in the expression regulation of basilic protein complexes in photosynthesis, such as PSI, PSII, Cytochrome b6-f complex, ATP synthase, and RuBisCO. Our results indicate that the inactivation of SRPS-enzymes have a significant influence on carbon sequestration by photosynthesis of Synechococcus sp. PCC7002.