Reduction in plasma vasopressin levels of dehydrated rats following acute stress.
Reduction in plasma vasopressin levels of dehydrated rats following acute stress.
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急性应激后脱水大鼠血浆加压素水平降低。
DOI：
10.1210/endo-100-1-30
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发表时间：
1977
期刊：
影响因子：
4.8
通讯作者：
W. Severs
中科院分区：
文献类型：
--
作者：
L. Keil;W. Severs
建立了一种灵敏的血浆精氨酸加压素(PAVP)放射免疫分析方法。用该方法测定了非脱水大鼠和脱水大鼠暴露于乙醚或加速应激后血浆中加压素的水平。同时测定尼古丁给药后大鼠的血浆AVP水平。非脱水大鼠暴露于乙醚1min后1、2、5、15min的pAVP无明显变化。另一方面，脱水的大鼠在暴露于乙醚后，pAVP显著减少。其中一组(180~220g)应激后2min下降27%(P<0.001)，5min下降47%(P<0.05)。在一组较大的动物(350-400g)中，在乙醚应激后1min，pAVP水平下降了55%(P<0.05)，2min时下降了72%(P<0.01)。第三组(250-300g)在乙醚应激后5min，pAVP值也显著降低，为57%(P<0.01)，但在15min时无明显变化。非脱水大鼠在-4.1Gx恒温处理5、15或120min后，pAVP无明显变化。脱水大鼠离心5min后，pAVP无明显下降，离心120min后，平均pAVP较未离心对照组下降40%(P<0.02)。与乙醚或加速应激不同，尼古丁在注射10min后可引起pAVP显著升高(P<0.005)。这些结果表明，乙醚或加速应激不会引起大鼠pAVP水平的升高，而且，在脱水大鼠中，这些应激源会引起pAVP水平的显著下降。
The development of a sensitive radioimmunoassay for plasma arginine vasopressin (pAVP) is described. Using this assay, the levels of vasopressin were determined in the plasma of nondehydrated and dehydrated rats after exposure to ether or acceleration stress. Plasma AVP was also determined in rats following nicotine administration. Nondehydrated rats showed no significant changes in pAVP 1, 2, 5, or 15 min after exposure to ether for 1 min. Dehydrated rats, on the other hand, had significantly reduced pAVP after exposure to ether. One group (180-220 g) showed a decline in pAVP of 27% at 2 min (P less than 0.05) and and 47% at 5 min (P less than 0.001) after stress. In a group of larger animals (350-400 g), pAVP levels were reduced by 55% at 1 min (P less than 0.05) and 72% at 2 min (P less than 0.01) after ether stress. A third group (250-300 g) also had significantly reduced pAVP values of 57% (P less than 0.01) 5 min after ether stress but not at 15 min. Nondehydrated rats which were centriguated at -4.1 Gx for 5, 15 or 120 min showed no significant alterations in pAVP. No decrease in pAVP was observed in dehydrated rats centrifugated for 5 min; after 120 min of centrifugation, mean pAVP was reduced by 40% (P less than 0.02) when compared to be noncentrifugated controls. In contrast to either ether or acceleration stress, nicotine provoked a marked rise (P less than 0.005) in pAVP 10 min after injection. From these results it was concluded that ether or acceleration stress does not evoke an increase in the pAVP levels of rats, and furthermore, in dehydrated rats, these stressors will produce a significant decline in pAVP.