Isolation and identification of pathogenic bacterium associated with ascites in Cynoglossus semilaevisis
Isolation and identification of pathogenic bacterium associated with ascites in Cynoglossus semilaevisis
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发表时间：
2015
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通讯作者：
Xue Xiaol
中科院分区：
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作者：
Xue Xiaol
从病鱼腹水和肾脏中分离到一株对半滑舌鳎有较强致病性的优势菌8301。通过人工挑战半滑舌鳎。对该菌的形态、生理生化特性进行了研究，并对16 S rDNA基因进行了部分序列测定，并与GenBank数据库中的序列进行了比较。结果表明，8301菌株为链状革兰氏阳性球菌，经生理生化特性分析，鉴定为海豚链球菌。16SrDNA系统发育分析表明，菌株8301与S. iniae，自举率为96%。这些结果表明，引起C. semilaevisis为S. iniae。药敏试验结果表明，8301菌株对制霉菌素、利福平、青霉素、阿奇霉素敏感，对罗红霉素、呋喃唑酮等耐药。
Dominant bacteria designed as 8301 was isolated from ascites and kidney of diseased Cynoglossus semilaevisis and proved to be pathogenic strongly to C. semilaevisis by artificial challenge. The biological characteristics including morphological, physiological and bio-chemical characteristics were examined, and the 16 S rDNA gene were partially sequenced and compared with sequences deposited in GenBank databases. The results showed that strain 8301 appeared gram-positive cocci in chains; based on the physiological and biochemical characteristic analysis, strain 8301 was identified as Streptococcus iniae. Phylogeny analysis with 16 S rDNA showed that strain 8301 was branched into the same cluster of S. iniae and the bootstrap was 96%. These results determined that the pathogen of causing ascites in C. semilaevisis was S. iniae. Antimicrobial susceptibility assay showed that strain 8301 was susceptible to nystatin, rifampicin, penicillin and azithromycin, but resistant to roxithromycin, furazolidone, etc.