ANALYSIS OF PCP-DATA TO DETERMINE FRACTION OF CELLS IN VARIOUS PHASES OF CELL-CYCLE
ANALYSIS OF PCP-DATA TO DETERMINE FRACTION OF CELLS IN VARIOUS PHASES OF CELL-CYCLE
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DOI：
10.1007/bf02339807
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发表时间：
1975-01-01
影响因子：
1.7
通讯作者：
LINDEN, WA
中科院分区：
文献类型：
--
作者：
BAISCH, H;GOHDE, W;LINDEN, WA
描述了用于脉冲细胞光度计（PCP）数据分析的数学模型。用基于这些模型的计算机程序，获得了G1-，S-和（G2+ M）-期细胞的分数。该方法适用于埃利希腹水瘤细胞、人骨髓细胞和L-929-细胞培养物的PCP测定。的L-细胞实验的结果进行了比较与放射自显影的结果;对于这两种方法的持续时间的各个阶段已经计算。两种不同的数学模型PCP数据评价和放射自显影方法产生的结果在统计误差范围内一致。讨论了两种模型在不同类型DNA直方图上的应用：一种模型适用于S期细胞比例低的异步细胞群体，另一种模型也适用于部分同步细胞和S期细胞比例高的群体。
Mathematical models for the analysis of pulse-cytophotometric (PCP) data are described. With computer programs based on these models the fractions of cells in G1-, S- and(G2+ M)-phases are obtained. The methods are applied to PCP measurements of Ehrlich ascites tumor cells, human bone marrow cells and L-929-cells in culture. The results of the L-cell experiment are compared with autoradiographic results; for both methods the duration of the various phases has been calculated. Two different mathematical models for PCP-data evaluation and the autoradiographic method yielded results agreeing within statistical error. The application of the two models on different types of DNA-histograms is discussed: One model is suitable for asynchronous cell populations with a low fraction of S-phase cells, the other can be applied for partially synchronized cells and high S-phase fractions as well.