Effect of age, smoking and other lifestyle factors on urinary 7‐methylguanine and 8‐hydroxydeoxyguanosine
Effect of age, smoking and other lifestyle factors on urinary 7‐methylguanine and 8‐hydroxydeoxyguanosine
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DOI：
10.1111/j.1349-7006.2009.01088.x
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发表时间：
2009-04
期刊：
影响因子：
5.7
通讯作者：
K. Tamae;K. Kawai;S. Yamasaki;K. Kawanami;M. Ikeda;Ken Takahashi;T. Miyamoto;Noritada Kato;H. Kasai
中科院分区：
文献类型：
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作者：
K. Tamae;K. Kawai;S. Yamasaki;K. Kawanami;M. Ikeda;Ken Takahashi;T. Miyamoto;Noritada Kato;H. Kasai
采用柱切换高效液相色谱法分别测定尿中8‐羟基脱氧鸟苷（8‐OH‐dG）和7‐甲基鸟嘌呤（m7Gua）作为氧化和甲基化DNA损伤的标志物。我们调查了尿8 - OH - dG或m7Gua与各种生活方式和人口因素（如年龄和性别）之间的关系。尿8 - OH - dG排泄水平与吸烟呈正相关，但与水果摄入量、体力活动和每日总能量消耗呈负相关。一项多元回归分析显示，日常体育活动和健康膳食组合降低了尿8 - OH - dG水平，而饮酒则增加了尿8 - OH - dG水平。在尿m7Gua测量中，吸烟、年龄、食用肉、鱼、蛋、大豆等与尿m7Gua水平呈正相关，而体重、BMI、体力活动、营养平衡良好的膳食指数得分与尿m7Gua水平呈负相关。多元回归分析发现吸烟和年龄与m7Gua水平相关，而高BMI和健康膳食组合对m7Gua水平有显著降低作用。因此，尿m7Gua水平被认为是DNA甲基化的有用标记，不仅与吸烟有关，还与衰老和不健康的饮食习惯有关。（癌症科学2009；100:715-721）
Urinary 8‐hydroxydeoxyguanosine (8‐OH‐dG) and 7‐methylguanine (m7Gua) were measured by a column‐switching high performance liquid chromatography method as markers of oxidative and methylating DNA damage, respectively. We investigated the associations between urinary 8‐OH‐dG or m7Gua and various lifestyle and demographic factors, such as age and sex. The urinary 8‐OH‐dG excretion level was positively correlated with cigarette smoking, but inversely correlated with fruit consumption, physical activity and total energy consumed per day. A multiple regression analysis revealed that daily physical activity and healthy meal combinations decreased the urinary 8‐OH‐dG level, whereas alcohol consumption increased it. In terms of the urinary m7Gua measurement, cigarette smoking, age and consumption of meat, fish, egg, soybean, etc. were positively correlated with the urinary m7Gua level, whereas body weight, BMI, physical activity, and dietary index score, which indicates good nutritional balance, were negatively correlated with the amount of m7Gua. Based on a multiple regression analysis, cigarette smoking and age correlated with the m7Gua level, while high BMI and healthy meal combinations have significant reducing effects on m7Gua level. Therefore, the urinary m7Gua level is considered to be a useful marker of DNA methylation, not only from smoking, but also from aging and unhealthy dietary habits. (Cancer Sci 2009; 100: 715–721)