Effect of Bizhongxiao decoction and its dismantled formulae on IL-1 and TNF levels in collagen-induced arthritis in rat synovial joints.
Effect of Bizhongxiao decoction and its dismantled formulae on IL-1 and TNF levels in collagen-induced arthritis in rat synovial joints.
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DOI：
10.1186/1742-4682-9-47
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发表时间：
2012-11-20
影响因子：
--
通讯作者：
Liang QH
中科院分区：
文献类型：
--
作者：
Guo YJ;Chen J;Xiong XG;Wu D;Zhu H;Liang QH
类风湿性关节炎 (RA) 是一种慢性自身免疫性疾病，以多种不同方式影响患者。这种慢性病的治疗尤其具有挑战性。传统中医 (TCM) 提供了替代方案。痹中消汤（BZX）是一种中药复方，在临床上已用于治疗RA多年。本研究的目的是比较BZX汤及其拆方对RA大鼠IL-1和TNF-1水平的影响，并阐明其作用机制。将90只健康正常雌性SD大鼠随机分为6组：正常组（对照组）、模型组、补中心汤组、三拆方（I组：清热解毒组、II组：祛湿组、III组：活血组）。除正常组（对照组）外，各组大鼠皮下注射牛II型胶原和弗氏完全佐剂建立胶原性关节炎模型，观察BZX汤及其拆方对大鼠足肿胀的抑制作用。采用免疫组织化学方法评估不同时间点滑膜关节中炎症细胞因子 IL-1 和 TNF 的水平。模型建立后21天，模型组、补中心汤组及拆方Ⅰ、Ⅱ、Ⅲ组TNF、IL-1水平均显着高于正常对照组（P<0.05）。模型组这些细胞因子的水平显着高于BZX汤组或三拆方组(P<0.01)。随着时间的延长，模型组TNF和IL-1水平逐渐升高； BZX汤剂组和拆方组逐渐减少。 BZX汤剂组细胞因子水平低于3个拆方组，并持续下降。在胶原性关节炎大鼠模型中，BZX汤剂和三个拆方均能下调炎症因子IL-1和TNF，但BZX的作用最强。
Rheumatoid arthritis (RA), a chronic autoimmune disease, affects sufferers in many different ways. Treatment of this chronic condition is particularly challenging. Traditional Chinese Medicine (TCM) provides alternatives. Bizhongxiao decoction (BZX) is a TCM complex, which has been used clinically for many years to treat RA. The purpose of this study is to compare the effects of BZX decoction and its dismantled formulae on IL-1 and TNF-1 levels in rats with RA, and to elucidate its mechanism of action. Ninety healthy normal female SD rats were randomly divided into six groups: normal (control), model, BZX decoction, and the three dismantled formulae (I: heat-clearing and detoxication, II: dissipating dampness, and III: blood circulation promotion). Apart from the normal (control) group, the rats in each group were injected subcutaneously with bovine type II collagen and complete Freund adjuvant to establish a collagen-induced arthritis model, so that inhibition of foot swelling in the rats by BZX decoction and its dismantled formulae could be observed. Immunohistochemistry was used to assess the levels of the inflammatory cytokines IL-1 and TNF in synovial joints at various time points. Twenty-one days after the model was established, the levels of TNF and IL-1 were significantly higher in the model group, BZX decoction group and dismantled formula groups I, II and III than in the normal controls (P < 0.05). The levels of these cytokines were significantly higher in the model group than the BZX decoction or the three dismantled formula groups (P <0.01). At longer times, the TNF and IL-1 levels in model group rose gradually; those in the BZX decoction and dismantled formula groups were gradually reduced. The cytokine levels in the BZX decoction group were lower than in the three dismantled formula groups and continued to decline. BZX decoction and the three dismantled formulae examined down-regulated the inflammatory factors IL-1 and TNF in collagen-induced arthritis rat models, but BZX exerted the strongest effect.
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影响因子：
27.4
作者：
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通讯作者：
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作者：
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影响因子：
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作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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