Morroniside promotes bone marrow mesenchymal stem cell proliferation in rats
Morroniside promotes bone marrow mesenchymal stem cell proliferation in rats
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DOI：
10.3892/mmr.2013.1399
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发表时间：
2013-05-01
影响因子：
3.4
通讯作者：
Zhao, Chengbin
中科院分区：
文献类型：
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作者：
Hu, Naiwu;Ren, Shujun;Zhao, Chengbin
本研究旨在探讨莫诺苷促进大鼠骨髓间充质干细胞（RMSC）增殖的机制，为潜在新药的开发提供实验依据。从3-4月龄的Sprague-Dawley大鼠的骨髓获得RMSC。光镜下观察高、中、低浓度莫诺苷干预组和空白对照组原代和传代培养的RMSCs增殖情况。MTT比色法检测细胞增殖和存活情况。光镜及MTT法检测显示莫诺苷组RMSC贴壁时间较对照组缩短。在原代RMSCs更换培养基后12小时，莫诺苷组中贴壁细胞的数量增加，观察到细长的细胞形态。莫诺苷组第4代细胞在接种后12-16 h完全融合，并迅速进入对数生长期。莫诺苷干预组的原代RMSCs经莫诺苷处理4 d后，已生长为典型的骨髓间充质干细胞集落，9-11 d融合率达80%。而对照组经莫诺苷处理14天后细胞融合率仅达75-80%。莫诺苷对原代和传代培养的骨髓间充质干细胞的增殖具有相似的促进作用。莫诺苷可通过分泌因子、细胞间相互作用和/或细胞粘附分子与细胞外基质（ECM）之间的相互作用促进RMSC增殖。然而，这种效应背后的具体机制仍有待充分阐明。
The aim of this study was to explore the mechanism underlying the promotive effect of morroniside on rat mesenchymal stem cell (RMSC) proliferation and to provide an experimental basis for the development of potential new drugs. RMSCs were obtained from the bone marrow of Sprague-Dawley rats aged 3-4 months. The proliferation of primary and subcultured RMSCs in the high-, medium- and low-concentration morroniside intervention and blank control groups was observed using light microscopy. Cell proliferation and survival conditions were detected using methyl thiazolyl tetrazolium (MTT) colorimetric tests. Light microscopy and the MTT assay revealed that RMSC adherence time in the morroniside groups was shorter compared with that of the control group. Twelve hours after the media of primary RMSCs were changed, the number of adherent cells in the morroniside groups increased and an elongated cell morphology was observed. The cells at the fourth passage in the morroniside groups fused completely 12-16 h after inoculation and then rapidly entered into the logarithmic phase. The primary RMSCs of the morroniside intervention groups grew into typical bone marrow mesenchymal stem cell (BMSC) colonies after 4 days of morroniside treatment and their fusion rate had reached 80% after 9-11 days. By contrast, the cell fusion rate of the control group only reached 75-80% after 14 days of morroniside treatment. Morroniside exhibited a similar promotive effect on proliferation in primary and subcultured RMSCs. Morroniside may promote RMSC proliferation through secreted factors, cell-to-cell interactions and/or the interactions between cellular adhesion molecules and extracellular matrices (ECMs). However, the specific mechanism underlying this effect remains to be fully elucidated.