Characterization of 7-alpha-dehydroxylase in Clostridium leptum.
Characterization of 7-alpha-dehydroxylase in Clostridium leptum.
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薄梭菌中 7-α-脱羟酶的表征。
DOI：
10.1093/ajcn/31.10.s243
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
P. Hylemon
中科院分区：
文献类型：
--
作者：
E. Stellwag;P. Hylemon
放射线层析分析表明，初生胆汁酸的7-α-脱羟化作用存在于薄梭菌的全细胞中，但在类杆菌属的肠道中未观察到。在pH为5~9的范围内检测到7-α-脱羟基酶的活性，在有0.1 mM胆酸钠存在的情况下，细胞培养物中的7-α-脱羟基酶比活性高8倍。2，4-二硝基苯酚、间氯苯肼和二环己基碳二亚胺对全细胞7-α-脱羟基酶活性有明显的抑制作用。提出了7-α-脱羟基肠道厌氧菌的饮食调控假说。
7-alpha-Dehydroxylation of primary bile acids was demonstrated radiochromatographically in whole cells of Clostridium leptum but was not observed in intestinal Bacteroides species. Activity of 7-alpha-Dehydroxylase was detected within a pH range of 5 to 9 and was 8-fold higher in specific activity in cell cultures in the presence of 0.1 mM sodium cholate than in its absence. 7-alpha-Dehydroxylase activity in whole cells was markedly inhibitied by 2,4-dinitrophenol, carbonyl-cyanide-m-chlorophenylhydrazine, and dicyclohexylcarbodiimide. A hypothesis concerning the dietary regulation of 7-alpha-dehydroxylating intestinal anaerobic bacteria is presented.