Activation of bone marrow-derived mesenchymal stromal cells a new mechanism of defocused low-energy shock wave in regenerative medicine
Activation of bone marrow-derived mesenchymal stromal cells a new mechanism of defocused low-energy shock wave in regenerative medicine
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DOI：
10.1016/j.jcyt.2013.08.012
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发表时间：
2013-12-01
期刊：
影响因子：
4.5
通讯作者：
Zhang, Haiyang
中科院分区：
文献类型：
--
作者：
Zhao, Yong;Wang, Jinbang;Zhang, Haiyang
背景目标。散焦低能量冲击波（DLSW）疗法已在再生医学中显示出有效性。其作用机制主要集中在创伤组织的病理生理改善上。在这项研究中，DLSW处理的干细胞的激活首先被视为愈合过程中的重要途径。方法.培养的大鼠骨髓间充质干细胞（BMSC）在每次传代前用DLSW处理。未处理的BMSC作为对照。采用酶联免疫法检测血管内皮生长因子（VEGF）和CXC配体5（CXCL 5）的分泌。流式细胞术分析第4代BMSC表面抗原（CD 166、CD 44和CD 34）的表达。Western blot检测增殖细胞核抗原（PCNA）和1（167）的表达。采用Matrigel毛细血管样管形成实验和大鼠盆大神经节培养实验检测休克和非休克BMSC条件培养液的愈合能力。结果休克组BMSC分泌VEGF和CXCL5明显高于未休克组。CD166、CD44、CD34在休克组与未休克组间无显著性差异（P > 0.05）。休克组BMSC增殖细胞核抗原（PCNA）和Ki67的表达明显高于未休克组（P < 0.01）。休克BMSC条件培养液能促进盆神经节神经突起的生长（P < 0.05）和基质胶内皮细胞管样结构的形成（P < 0.05）。结论. DLSW不干扰细胞表面标志物的表达。DLSW可促进BMSC的分泌和增殖，促进血管生成和神经再生。
Background aims. Defocused low-energy shock wave (DLSW) therapy has shown effectiveness in regenerative medicine. The mechanism of action was mainly focused on the pathophysiological improvement at the wound tissues. In this study, the activation of stem cells treated by DLSW was first examined as an important pathway during the healing process. Methods. Cultured rat bone marrow-derived mesenchymal stromal cells (BMSC) were treated by DLSW before each passage. The untreated BMSC served as a control. The secretions of vascular endothelial growth factor (VEGF) and CXC ligand 5 (CXCL5) were tested by means of enzyme-linked immunoassay. Flow cytometry was performed to analyze the BMSC (passage 4) surface antigen expressions (CD 166, CD44 and CD34). The expressions of proliferating cell nuclear antigen and 1(167 were analyzed by means of Western blot. The healing abilities of conditioned media of shocked and unshocked BMSC were examined by Matrigel-based capillary-like tube formation assay and rat major pelvic ganglia culture test. Results. The shocked BMSC secreted more VEGF and CXCL5 than did those of unshocked BMSC. The expressions of CD 166, CD44 and CD34 showed no significant differences (P > 0.05) between the shocked and unshocked BMSC. The shocked BMSC demonstrated higher expressions of proliferating cell nuclear antigen (P < 0.01) and Ki67 (P < 0.01) than did those of unshocked BMSC. The shocked BMSC conditioned medium showed higher ability to enhance the growth of major pelvic ganglia neurites (P < 0.05) and Matrigel-based endothelial tube-like formation (P < 0.05). Conclusions. DLSW did not interfere with the expressions of cell surface markers. DLSW enhanced the secretion and proliferation of BMSC and promoted angiogenesis and nerve regeneration in vitro.